oriTDB

The information of the oriT region

oriTDB accession number	100596
Name	oriT_pC49-108
Sequence Completeness	intact
oriT length	110 nt
oriT sequence	TCACTTCAGGCTCCTTACGGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATA GCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTCAGG
IR (inverted repeat)	IR1: [24-31] [35-42] nt (GTCGGGGC..GCCCTGAC) IR2: [49-65] [72-88] nt (GCAATTGTAATAGCGTC..GACGGTATTACAATTGC)
Location of nic site	[96-97] nt
Conserved sequence flanking the nic site	CATCCTG\|T
Note	predicted by the oriTfinder
	L:24-31;R:35-42;L:49-65;R:72-88;D:96-97

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TCACTTCAGGCTCCTTACGGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATA

61

GCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTCAGG

Reference
N/A

I. Information of Relaxase

ID	668
Name	Putative relaxase of pC49-108
GenBank accession number	YP_009069048
Family	MOB_P
Length	899 aa
UniProt ID	_
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 9.20E-64, Aligned region: 47..315
Note	putative relaxase
Protein sequence [Download]	MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRL RNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHY ILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYL NRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRIVRKTAVERDRQNAWTRGKKQTF REYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGKFLVMDGWDRNREGVQLDSFG PSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETESLQQYACRHL GERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKREL FSDKESLWGYSISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHE QTPVKASSIHPDLTLGRAEPQAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDP MLRRQRREARAEARADLRARYLAWREQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDP ALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVS QLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGE FVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNNDASLPPPEQDKRWEPPSPG

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1056
Plasmid name	pC49-108
GenBank accession number	NC_025144.1
Incompatibility group	IncI1
Genome size	108660 bp
Coordinate of oriT [Strand]	54100..54209 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli Chicken-49 [562]

Reference

[1] Wang J et al (2014) Nucleotide sequences of 16 transmissible plasmids identified in nine multidrug-resistant Escherichia coli isolates expressing an ESBL phenotype isolated from food-producing animals and healthy humans. J Antimicrob Chemother. 69(10):2658-68. [PMID:24920651]