oriTDB

The information of the oriT region

oriTDB accession number	100589
Name	oriT_pPBA
Sequence Completeness	intact
oriT length	188 nt
oriT sequence	GGGGTAGGTATCTCCCAACCGTGAAGCGCGATCCAATGGGATTGAGCGTAACGGTTAACA TTAGCCGCCGCAAGGCTTATCCTGCCATACAGTAGATACCTACCCCACAAACAAAAAGCG GTGGCAACTGCCACCGCTCTAAATTCCACATCGCCTCGATGCTGAGTGAACGTGGACGGT AAAAGCTA
IR (inverted repeat)	IR1: [17-36] [37-56] nt (AACCGTGAAGCGCGATCCAA..TGGGATTGAGCGTAACGGTT) IR2: [115-126] [129-140] nt (AAAGCGGTGGCA..TGCCACCGCTCT)
Location of nic site	[84-86] nt
Conserved sequence flanking the nic site	TATCCTG\|C
Note	predicted by the oriTfinder
	L:19-38;R:39-58;L:117-128;R:131-142;D:86-87

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGGGTAGGTATCTCCCAACCGTGAAGCGCGATCCAATGGGATTGAGCGTAACGGTTAACA

61

TTAGCCGCCGCAAGGCTTATCCTGCCATACAGTAGATACCTACCCCACAAACAAAAAGCG

121

GTGGCAACTGCCACCGCTCTAAATTCCACATCGCCTCGATGCTGAGTGAACGTGGACGGT

181

AAAAGCTA

Reference
N/A

I. Information of Relaxase

ID	661
Name	MobA/VirD2_pPBA
GenBank accession number	YP_009067864
Family	MOB_P
Length	347 aa
UniProt ID	A0A059U2Z1
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 2.80E-06, Aligned region: 72..262
Note	putative relaxase
Protein sequence [Download]	MSDFDSSFEAGQLAALFKPKLTSDPNRRGADMLLRSLSSRRAGQGGSTRARLARVVSRAP EVMVKVTGRPKGKNHAAAHFDYIGRKGDVPLETRDGDILTDKEDRAELARDWGDPVYWRD NSTVAAVSMVFSMPSGTDPDKVLSAVREVARSEIGHEWDYVLALHTDTPRPHVHVTVAAR GDTGRRFNPRPQTLHHYRERFAEELRARGVTAEATPRAARGVGRAGQSMALNRMRQRYMA GTAPAPFANQKITSAARDQLAGRSTAPDFVVRGRQAWNETHRRYLAAAKRLEASSDPADR QLADQVREFVGAGRTPTIHERSVAAIERKRQNERSRDRDRSREGPER

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1049
Plasmid name	pPBA
GenBank accession number	NC_025133.1
Genome size	59859 bp
Coordinate of oriT [Strand]	57279..57466 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Sphingobium wenxiniae JZ-1 [595605]

Reference

[1] Wang C et al (2014) A novel angular dioxygenase gene cluster encoding 3-phenoxybenzoate 1',2'-dioxygenase in Sphingobium wenxiniae JZ-1. Appl Environ Microbiol. 80(13):3811-8. [PMID:24747891]