oriTDB
The information of the oriT region
oriTDB accession number 100589
Name oriT_pPBA  insolico
Sequence Completeness intact
oriT length 188 nt
oriT sequence GGGGTAGGTATCTCCCAACCGTGAAGCGCGATCCAATGGGATTGAGCGTAACGGTTAACA TTAGCCGCCGCAAGGCTTATCCTGCCATACAGTAGATACCTACCCCACAAACAAAAAGCG GTGGCAACTGCCACCGCTCTAAATTCCACATCGCCTCGATGCTGAGTGAACGTGGACGGT AAAAGCTA
IR (inverted repeat)IR1: [17-36] [37-56] nt  (AACCGTGAAGCGCGATCCAA..TGGGATTGAGCGTAACGGTT)
IR2: [115-126] [129-140] nt  (AAAGCGGTGGCA..TGCCACCGCTCT)
Location of nic site [84-86] nt
Conserved sequence flanking the
  nic site 		TATCCTG|C
Note predicted by the oriTfinder
L:19-38;R:39-58;L:117-128;R:131-142;D:86-87
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 661
Name MobA/VirD2_pPBA insolico
GenBank accession number YP_009067864
Family MOBP
Length 347 aa
UniProt ID A0A059U2Z1
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 2.80E-06, Aligned region: 72..262
Note putative relaxase
Protein sequence [Download] MSDFDSSFEAGQLAALFKPKLTSDPNRRGADMLLRSLSSRRAGQGGSTRARLARVVSRAP
EVMVKVTGRPKGKNHAAAHFDYIGRKGDVPLETRDGDILTDKEDRAELARDWGDPVYWRD
NSTVAAVSMVFSMPSGTDPDKVLSAVREVARSEIGHEWDYVLALHTDTPRPHVHVTVAAR
GDTGRRFNPRPQTLHHYRERFAEELRARGVTAEATPRAARGVGRAGQSMALNRMRQRYMA
GTAPAPFANQKITSAARDQLAGRSTAPDFVVRGRQAWNETHRRYLAAAKRLEASSDPADR
QLADQVREFVGAGRTPTIHERSVAAIERKRQNERSRDRDRSREGPER
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1049
Plasmid name pPBA
GenBank accession number NC_025133.1
Genome size 59859 bp
Coordinate of oriT  [Strand] 57279..57466 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Sphingobium wenxiniae JZ-1 [595605]
Reference
[1] Wang C et al (2014) A novel angular dioxygenase gene cluster encoding 3-phenoxybenzoate 1',2'-dioxygenase in Sphingobium wenxiniae JZ-1. Appl Environ Microbiol. 80(13):3811-8. [PMID:24747891]

Microbial Bioinformatics Group in MML, SJTU