oriTDB

oriT
Relaxosome
T4CP
Plasmid&Host

The information of the oriT region

oriTDB accession number	100582
Name	oriT_pCY-MdT
Sequence Completeness	intact
oriT length	185 nt
oriT sequence	GGTTTTAGCAGGTTTCGGGGCGCAGCCCTGAACCAGTCACCTAGCGCTAGCGGAGTGTAT ACTGGCTTAGTATGTTACGGCGAGATGGAGATTCAGGAAAGTGCTTCATGTGAGAGGGGG AAAATGCTGCTGCAGGGAGTAGGTGCGAAATGCCGGTGTCGGTGACATGACGCCTCCTCG CTCAC
IR (inverted repeat)	[14-21] [25-32] nt (TTCGGGGC..GCCCTGAA)
Location of nic site	[65-66] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:14-21;R:25-32;D:65-66

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	654
Name	MobA_pCY-MdT
GenBank accession number	YP_009062813
Family	MOB_P
Length	516 aa
UniProt ID	A0A076UB37
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 3.70E-17, Aligned region: 58..264
Note	putative relaxase
Protein sequence [Download]	MPDSSGCGMPCWKRVQTMIVKFHPRGRGGGAGPVDYLLGKDRQRDGATVLQGKPEEVREL IDASPYAKKYTSGVLSFAEKDLPPGQREKLMASFERVLMPGLDKDQYSVLWVEHQDKGRL ELNFLIPNMELLTGRRLQPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSAL PKTKQEAAQAITRGLLALASSGELKTRQDVTEALESAGFEVVRTTKSSISIADPDGGRNI RLKGAIYEQSFNAGKGLRAEIESAAAEYRRDAESRIQRAREVCQSGTERKREENQRRHPR PRPEAVLSHEPAHERHGAYGQPDVADHRPGVYTADSIGRKHSMVAGAADTRQLHDHPGAE EHAGHAVREEQRRAALDLRRAETPLREGEPGSGTVRRGFELDDTGGEIAHDGAGKTVAER IRAATAGLLEKAGRVGERLRGMADDVWSYATGERGAERARHGLEQAGAEFERAAAPVVTR LNVIEAHREQERAAQHQKALELERSQRQRTYNGPSL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1042
Plasmid name	pCY-MdT
GenBank accession number	NC_025003.1
Genome size	5933 bp
Coordinate of oriT [Strand]	1969..2153 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Enterobacter cloacae CY01 [550]

Reference

[1] Yang L et al (2014) resistome analysis of Enterobacter cloacae CY01, an extensively drug-resistant strain producing VIM-1 metallo-β-lactamase from China. Antimicrob Agents Chemother. 58(10):6328-30. [PMID:25114139]