oriTDB
The information of the oriT region
oriTDB accession number 100580
Name oriT_pAAA83  insolico
Sequence Completeness intact
oriT length 373 nt
oriT sequence GCCTCGCCTCGTCTCTCATGCCGGCGGTAGCCGGCTGCCTCGCAGAGCAGGATGACCCGT TGAGCGCCCCCGGCGCGAATAAGGGACAGTGAAGATAGATAACCGGCTCGCCGGTTAGCT AACTTCACACATCCTGCCCGCCTTACGGCGTTAATAACACCAAGGAAAGTCTACACCAGC CATTACGATTTATCCGCAACTATCGCGCTATCAGGCCGCAAAAGCAGCAACGGATATAGC GAAACCCGCCACAATGGCCCATAATGCCGCTATCGAAGCGTGCCAATGCACGCCGATAGC GGACTTTTTGCGTTTCCGTAGCGCCGCTTAGTAGCGTTACATTTGCGATGAGAGGATTAG ATGGACGAACACG
IR (inverted repeat)IR1: [64-68] [72-76] nt  (GCGCC..GGCGC)
IR2: [89-107] [110-128] nt  (GTGAAGATAGATAACCGGC..GCCGGTTAGCTAACTTCAC)
Location of nic site [136-137] nt
Conserved sequence flanking the
  nic site 		CATCCTG|C
Note predicted by the oriTfinder
L:64-68;R:72-76;L:89-107;R:110-128;D:136-137
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 652
Name Relaxase_pAAA83 insolico
GenBank accession number YP_009062664
Family MOBP
Length 746 aa
UniProt ID A0A077KSK4
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 9.90E-64, Aligned region: 12..252
Note putative relaxase
Protein sequence [Download] MIAKHVPMRSLGKSDFAGLANYITDAQSKDHRLGHVQATNCEAGSIQDAITEVLATQHTN
TRAKGDKTYHLIVSFRAGEQPSADTLRAIEERICVGLGYGEHQRISAVHNDTDNLHIHIA
INKIHPTRHTMHEPYYPHRALAELCTALERDYGLERDNHEPRKRGAEGRAADMERHAGVE
SLVGWIKRECLDEIKGAQSWQELHQVMRDNGMELRVRANGLVFEAGDGTMVKASTVARDL
SKPSLEARLGPFEASPERQAQTTAKRQYRKDPIRLRVNTVELYAKYKAEQQSLTTARAQA
LERARHRKDRLIEAAKRSNRLRRATIKVVGEGRANKKLLYAQASKALRSEIQAINKQFQQ
ERTALYAEHSRRTWADWLKKEAQHGGADALAALRAREAAQGLKGNTIRGEGQAKPGHAPA
VDNITKKGTIIFRAGMSAVRDDGDRLQVSREATREGLQEALRLAMQRYGNRITVNGTVEF
KAQMIRAAVDSQLPITFTDPALESRRQALLNKENTHERTERPEHRGRTGRGAGGPGQRPA
ADQHATGAAAVARAGDGRPAAGRGDRADAGLHAATVHRKPDVGRLGRKPPPQSQHRLRAL
SELGVVRIAGGSEVLLPRDVPRHVEQQGTQPDHALRRGISRPGTGVGQTPPGVAAADKYI
AEREAKRLKGFDIPKHSRYTAGDGALTFQGTRTIEGQALALLKRGDEVMVMPIDQATARR
LTRIAVGDAVSITAKGSIKTSKGRSR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1040
Plasmid name pAAA83
GenBank accession number NC_024998.1
Incompatibility group IncP(Beta)
Genome size 59534 bp
Coordinate of oriT  [Strand] 9191..9563 [-]
Drug resistance insolico kasugamycin resistance; streptomycin resistance
Heavy-metal resistance insolico mercury resistance
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Acidovorax avenae subsp. avenae 83 [80870]
Reference
[1] Yoshii A et al (2015) Two types of genetic carrier, the IncP genomic island and the novel IncP-1β plasmid, for the aac(2')-IIa gene that confers kasugamycin resistance in Acidovorax avenae ssp avenae. Mol Plant Pathol. 16(3):288-300. [PMID:25131295]

Microbial Bioinformatics Group in MML, SJTU