 oriTDB accession number | 100579 |
| Name | oriT_pACM1  |
Sequence Completeness  | core |
| oriT length | 106 nt |
| oriT sequence | AGATAGCTAACCTCGTTAGGGGGTGTCGGGGCTTGCCCTGACCAAGACGTTTTTGGACGG CCGCCGCGTGTCGGCGGTACTCCAAAAACACATCTTGTCCCGTACT |
| IR (inverted repeat) | [18-35] [41-58] nt (GTTTTTGGAGTACCGCCG..CGGCGGCCGTCCAAAAAC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site | _ |
| Note | predicted by the oriTfinder |
Visualization of oriT structure Reference| ID | 651 |
| Name | Putative relaxase of pACM1 |
| GenBank accession number | YP_009062617 |
| Family | MOBP |
| Length | 658 aa |
| UniProt ID | A0A088FMU5 |
| PDB ID | _ |
| Pfam | Relaxase [PF03432.13], Evalue: 3.10E-31, Aligned region: 12..333 |
| Note | putative relaxase |
| Protein sequence [Download] | MIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRK QKVISTPVDVEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWP DYEKPNDDQIFDSVKFTLASMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSF TKDTLHQACRLLELKNGWSHSNGAYVVNDRQQIVRNPHSKKERGNWRSLDRINKMENKEG VETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHKTFADIGLRVEKAQGKKGYVIT HEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTAYTPGAYRRDAN KRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSVS DPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALS QMRGFAYSSRKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTV IFERDGKPVAADRGHIVLTESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNR IVDAAVNHNHPVAQGITFTDAAQQAYAQNEKHRLIREQNNSKNEMQFRSESDDKFNPK |
Reference| ID | 1039 |
| Plasmid name | pACM1 |
| GenBank accession number | NC_024997.1 |
| Incompatibility group | IncL/M(pMU407) |
| Genome size | 89977 bp |
| Coordinate of oriT [Strand] | 64881..64986 [+] |
| Drug resistance | tetracycline resistance; sulfonamide resistance; gentamicin resistance; tobramycin/amikacin resistance; trimethoprin resistance; spectinomycin resistance |
| Heavy-metal resistance | mercury resistance |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Klebsiella oxytoca [571] |
Reference| [1] Preston KE et al (2004) The SHV-5 extended-spectrum beta-lactamase gene of pACM1 is located on the remnant of a compound transposon. Plasmid. 51(1):48-53. [PMID:14711529] |
| [2] Preston KE et al (2002) Chromosomal sequences from Klebsiella pneumoniae flank the SHV-5 extended-spectrum beta-lactamase gene in pACM1. Plasmid. 48(1):73-6. [PMID:12206758] |
| [3] Preston KE et al (2000) Nucleotide sequence of a 7-kb fragment of pACM1 encoding an IncM DNA primase and other putative proteins associated with conjugation. Plasmid. 44(1):12-23. [PMID:10873523] |
| [4] Preston KE et al (1999) The cassettes and 3' conserved segment of an integron from Klebsiella oxytoca plasmid pACM1. Plasmid. 42(2):104-14. [PMID:10489327] |
| [5] Preston KE et al (1997) The resistance and integrase genes of pACM1, a conjugative multiple-resistance plasmid, from Klebsiella oxytoca. Plasmid. 37(2):105-18. [PMID:9169202] |