oriTDB
The information of the oriT region
oriTDB accession number 100569
Name oriT_pHNAH4-1  insolico
Sequence Completeness intact
oriT length 110 nt
oriT sequence TCACTTCAGGCTCCTTACGGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATA GCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTCAGG
IR (inverted repeat)IR1: [24-31] [35-42] nt  (GTCGGGGC..GCCCTGAC)
IR2: [49-65] [72-88] nt  (GCAATTGTAATAGCGTC..GACGGTATTACAATTGC)
Location of nic site [96-97] nt
Conserved sequence flanking the
  nic site 		CATCCTG|T
Note predicted by the oriTfinder
L:24-31;R:35-42;L:49-65;R:72-88;D:96-97
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 641
Name NikB_pHNAH4-1 insolico
GenBank accession number YP_009060040
Family MOBP
Length 871 aa
UniProt ID A0A023UG69
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 8.70E-64, Aligned region: 19..287
Note putative relaxase
Protein sequence [Download] MTDEELNLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFH
NCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVRHTLKSLGL
ADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNG
CWVHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLA
EDGLYLSQMDGKFLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEA
PGRYNPDFIAADVRPEKIAETESLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGL
WMRVQHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWQPVPTDIFRQVTPTERF
RGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEF
TWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFVSA
PADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQW
RKPDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDE
RQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCE
PGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLI
APVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITRPDVDHHREGSER
YYRDYIAANSNDDASLPPPEQDKRWEPPSPG
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1029
Plasmid name pHNAH4-1
GenBank accession number NC_024955.2
Incompatibility group IncI1
Genome size 109191 bp
Coordinate of oriT  [Strand] 55669..55778 [-]
Drug resistance insolico erythromycin resistance
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli AHC4 [562]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU