oriTDB
The information of the oriT region
oriTDB accession number 100566
Name oriT_pKP1-NDM-1  insolico
Sequence Completeness intact
oriT length 551 nt
oriT sequence GAGCAGAGCTATGTGTGACAAGAAGTATAGAAATTACGAGGTAGCCATCATGGTCGATGT GAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACAT CCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTG CTACATCACAGACGGGATTAAGGCTAATCAGGAGCCTGTGATTTACCCGATCATTGAAGA AGCTCTGCATCGCTACAGCCAGCTCGTGTTTCATGAGCAGAGAGAGAAATATGAAGACCC GGCCAGAATTGGGGCATTTCTGGAAACCCTGATCACCGAAACCTGCCGGGCGTTGGAAGT GCAAATTGTCGATAGTGGCGGTGATTCATGGTCTGTCGATTCAGGAGAGTCGTTCTCACT GTGGCTTTCTTCCCATCCAGGAGAACTATCCATTAACCCGCAGCCCCATGAGGATGAGAC CTCTTTGCGTGGCTTGCTGTATGAGCTCATCACCTGTGAGAGCGTGAAAACTGTTTTAAG GAGAACCGACT
IR (inverted repeat)IR1: [62-70] [71-79] nt  (AACCCTTTC..GACAGGGTT)
IR2: [143-150] [154-161] nt  (TGGCCTGC..GGAGGCCA)
Location of nic site _
Conserved sequence flanking the
  nic site 		_
Note predicted by the oriTfinder
L: 62-70;R:71-79;L:143-150;R:154-161
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 638
Name TraI_pKP1-NDM-1 insolico
GenBank accession number YP_009023007
Family MOBH
Length 990 aa
UniProt ID G9FJJ6
PDB ID _
Pfam TraI_2 [PF07514.10], Evalue: 6.50E-102, Aligned region: 44..362
Note putative relaxase
Protein sequence [Download] MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAEL
IEKVRNSLGFTVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQ
ASESVIFSIEGTPRERRDNEPRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSES
LHDWAHRHEIDRYFIRWRDKRHKRHEQFSLLAVDRIIPAETREFLSKSGPSIMEAMLEAI
SGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVPVERYVFDAIRRLVKTGKWKV
NEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIERGFAVPNTVQE
KGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKD
AGSTESSKPDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNP
LQALKDVGGGLGDIDFPFDAFSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQN
SLQGDDFPMFGSSDEPPSWAIEPLPMLTDAPEQTTPAPAMPPTDKPNLHEKDAKTLLVEM
LAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILYPDGARSLGAPSEVLSKLSHA
NAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDAFELVSPPGLD
ASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDIN
AEDFLPSGVTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISK
HILCGMLSRAQRRPLLKKRQGKLYLEVNET
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1026
Plasmid name pKP1-NDM-1
GenBank accession number NC_023908.1
Incompatibility group IncA/C2
Genome size 137538 bp
Coordinate of oriT  [Strand] 48617..49167 [+]
Drug resistance insolico aminoglycoside resistance; bleomycin resistance
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Klebsiella pneumoniae KP1 [573]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU