oriTDB
The information of the oriT region
oriTDB accession number 100542
Name oriT_pCOL-1  insolico
Sequence Completeness intact
oriT length 309 nt
oriT sequence CGTGTCTCCTTCTCGCCGGGGCGGGGGGCGGGGTGTGTCTTGGACAGTAAGAAGCGAGAG CGCCGCACTGTCCGGGGTCAAGGGGATACCCCTTGCAGGATAAGCCAGACCGTGAGAAGC GAAGCGCCGAATGGGCAGGCGTCCGTTGGAACCACGTGGCGAAGCCATGTGTGTTACAAC GGTCATCCTGTATTGCTCATCCGCTTTACTATCGTATCAGCAGTTTAAGTGCACGGGACG CACTTTGCAACAAGCACAAATGGCGCTATGCTGTCAACTCCGCAACAAGTAAGCAAAGGA GTTAGCACG
IR (inverted repeat)IR1:[79-85] [89-95] nt  (CAAGGGGC..GCCCCTTG)
IR2: [153-160] [164-171] nt  (CACGTGGC..GCCATGTG)
Location of nic site [190-191] nt
Conserved sequence flanking the
  nic site 		CATCCTG|T
Note predicted by the oriTfinder
L:79-85;R:89-95;L:153-160;R:164-171;D:190-191
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 613
Name MobA_pCOL-1 insolico
GenBank accession number YP_008532734
Family MOBP
Length 833 aa
UniProt ID T2AC20
PDB ID _
Pfam RepB_primase [PF16793.4], Evalue: 3.50E-70, Aligned region: 491..750
Relaxase [PF03432.13], Evalue: 1.00E-27, Aligned region: 6..217
Note putative relaxase
Protein sequence [Download] MIALSQEAVRSKDTINHYVLSWREGEQPSPEQVEEAVSIFMDELGVKDHQAIYGLHADTD
NLHLHLAINRVHPETLKVVKINNGFDIEAAHKAIARIENAQGWQREQNGRYQVLENGELG
REHIDKDKPRQPAQPKRDMENRTGEKSAERIAIEDGAPIIKKAQTWEQLHRELAAKGMRY
EKTGSGATLFVGDVGVKASSADRDASLSKLQKRLGAYQPPPQRQQVAQREPEPIKPDVPG
WKDYITGRKAHYAEKNAAKLALDKRQEQERKQIAEQQKARRDELMRGNWKGKGEVLNAMR
SVIAAEQAAEKAALKEKHQKQREQHRQQFRPYPDLEQWQRMQKSPELAEQWRHRASEPQR
IEGASGEPPTPRDIRAYQPEIVGQQVHYSRKEEAGAGGGVSFVDKGKSIDIHDWRNRDST
LAALQLSAQKWGSFTVTGNDEYKAMCAKLAAEHGFKITNPELQERIQQERQRIQQERAQA
MKSEQLKQFELYAEAVGAERYRVTSIKMQADGRKQTFILDKKDGITRGFTPQEIEQRTPE
MLRLQRRGENLYYTPLSDKKHHILIDDMNREKLERLIRDGYRPAVVLESSPGNYQAIITV
PKLGTAHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENRKPKHQREDGSYPEVRLL
KAERRECVKALALSSQIDAEYQRQAALKAQQPERSKAKPALELAAASGSAIDAYQRHYRD
VIKRQRGGEVDLSRVDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKDEGRDWNDYAQRT
ARYAYSAAGDRQAAELGKYRQQWEKLEGREPVRQQEQAKAQKIERDNSPGMSR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1002
Plasmid name pCOL-1
GenBank accession number NC_022346.1
Incompatibility group IncP(6)
Genome size 31529 bp
Coordinate of oriT  [Strand] 25086..25394 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Pseudomonas aeruginosa ST308 [287]
Reference
[1] Naas T et al (2013) Complete sequence of two KPC-harbouring plasmids from Pseudomonas aeruginosa. J Antimicrob Chemother. 68(8):1757-62. [PMID:23569197]

Microbial Bioinformatics Group in MML, SJTU