oriTDB

The information of the oriT region

oriTDB accession number	100542
Name	oriT_pCOL-1
Sequence Completeness	intact
oriT length	309 nt
oriT sequence	CGTGTCTCCTTCTCGCCGGGGCGGGGGGCGGGGTGTGTCTTGGACAGTAAGAAGCGAGAG CGCCGCACTGTCCGGGGTCAAGGGGATACCCCTTGCAGGATAAGCCAGACCGTGAGAAGC GAAGCGCCGAATGGGCAGGCGTCCGTTGGAACCACGTGGCGAAGCCATGTGTGTTACAAC GGTCATCCTGTATTGCTCATCCGCTTTACTATCGTATCAGCAGTTTAAGTGCACGGGACG CACTTTGCAACAAGCACAAATGGCGCTATGCTGTCAACTCCGCAACAAGTAAGCAAAGGA GTTAGCACG
IR (inverted repeat)	IR1:[79-85] [89-95] nt (CAAGGGGC..GCCCCTTG) IR2: [153-160] [164-171] nt (CACGTGGC..GCCATGTG)
Location of nic site	[190-191] nt
Conserved sequence flanking the nic site	CATCCTG\|T
Note	predicted by the oriTfinder
	L:79-85;R:89-95;L:153-160;R:164-171;D:190-191

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CGTGTCTCCTTCTCGCCGGGGCGGGGGGCGGGGTGTGTCTTGGACAGTAAGAAGCGAGAG

61

CGCCGCACTGTCCGGGGTCAAGGGGATACCCCTTGCAGGATAAGCCAGACCGTGAGAAGC

121

GAAGCGCCGAATGGGCAGGCGTCCGTTGGAACCACGTGGCGAAGCCATGTGTGTTACAAC

181

GGTCATCCTGTATTGCTCATCCGCTTTACTATCGTATCAGCAGTTTAAGTGCACGGGACG

241

CACTTTGCAACAAGCACAAATGGCGCTATGCTGTCAACTCCGCAACAAGTAAGCAAAGGA

301

GTTAGCACG

Reference
N/A

I. Information of Relaxase

ID	613
Name	MobA_pCOL-1
GenBank accession number	YP_008532734
Family	MOB_P
Length	833 aa
UniProt ID	T2AC20
PDB ID	_
Pfam	RepB_primase [PF16793.4], Evalue: 3.50E-70, Aligned region: 491..750 Relaxase [PF03432.13], Evalue: 1.00E-27, Aligned region: 6..217
Note	putative relaxase
Protein sequence [Download]	MIALSQEAVRSKDTINHYVLSWREGEQPSPEQVEEAVSIFMDELGVKDHQAIYGLHADTD NLHLHLAINRVHPETLKVVKINNGFDIEAAHKAIARIENAQGWQREQNGRYQVLENGELG REHIDKDKPRQPAQPKRDMENRTGEKSAERIAIEDGAPIIKKAQTWEQLHRELAAKGMRY EKTGSGATLFVGDVGVKASSADRDASLSKLQKRLGAYQPPPQRQQVAQREPEPIKPDVPG WKDYITGRKAHYAEKNAAKLALDKRQEQERKQIAEQQKARRDELMRGNWKGKGEVLNAMR SVIAAEQAAEKAALKEKHQKQREQHRQQFRPYPDLEQWQRMQKSPELAEQWRHRASEPQR IEGASGEPPTPRDIRAYQPEIVGQQVHYSRKEEAGAGGGVSFVDKGKSIDIHDWRNRDST LAALQLSAQKWGSFTVTGNDEYKAMCAKLAAEHGFKITNPELQERIQQERQRIQQERAQA MKSEQLKQFELYAEAVGAERYRVTSIKMQADGRKQTFILDKKDGITRGFTPQEIEQRTPE MLRLQRRGENLYYTPLSDKKHHILIDDMNREKLERLIRDGYRPAVVLESSPGNYQAIITV PKLGTAHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENRKPKHQREDGSYPEVRLL KAERRECVKALALSSQIDAEYQRQAALKAQQPERSKAKPALELAAASGSAIDAYQRHYRD VIKRQRGGEVDLSRVDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKDEGRDWNDYAQRT ARYAYSAAGDRQAAELGKYRQQWEKLEGREPVRQQEQAKAQKIERDNSPGMSR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1002
Plasmid name	pCOL-1
GenBank accession number	NC_022346.1
Incompatibility group	IncP(6)
Genome size	31529 bp
Coordinate of oriT [Strand]	25086..25394 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Pseudomonas aeruginosa ST308 [287]

Reference

[1] Naas T et al (2013) Complete sequence of two KPC-harbouring plasmids from Pseudomonas aeruginosa. J Antimicrob Chemother. 68(8):1757-62. [PMID:23569197]