oriTDB
The information of the oriT region
oriTDB accession number 100539
Name oriT1_pBBKW-1  insolico
Sequence Completeness core
oriT length 32 nt
oriT sequence ATGCAACCTCCGGTTGCATGTAAGTGCGCCCT
IR (inverted repeat)[1-8] [12-19] nt  (ATGCAACC..GGTTGCAT)
Location of nic site [28-29] nt
Conserved sequence flanking the
  nic site
TAAGTGCG|CCCT
Note predicted by the oriTfinder
L:1-8;R:12-19;D:28-29
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 610
Name Mob_pBBKW-1 insolico
GenBank accession number YP_008327316
Family MOBQ
Length 565 aa
UniProt ID S6BIT5
PDB ID _
Pfam MobA_MobL [PF03389.14], Evalue: 8.90E-66, Aligned region: 17..254
Note putative relaxase
Protein sequence [Download] MAIYHLSVSNVSRASGSKATATLSYISGKRVHDERRGETYDYGRKERVLRVGTLLPEGAP
AEYADPAVLFNAVELHETGRTARPAKKIVVALPREFTPRQRVQALEEYIRENLNADGYAA
TYAIHEDREGNNPHAHILVANRQIDPATGGWARLKQRMEYVLDERGERVPLIDPETGRQK
TDKRGRRQWKRTSVSLNPLDRKAKLKALRESWANTCNARLDETARIDHRSLEDQGSDLEP
TIHEGYAARAIERAGGVSERCQTNREIRRSNSLLTAVRAELGRIFDRLGELFAAKIRQLR
QRQARPEPAREPNWRYFEGDARRQLEADKADHTAAIRGKLMDARADVGNREEWWRSHGTE
EHEAAKQIIDVARAASREARDANIFKRGRLLRNAEQVAGEQSEKLRGAVPWLEDTDIPAD
WDQANRFRMRTTKAIHDHDMQPRTRLVAELERRLEQAERAESEKPSPEQVKALALRMAEQ
RAQAENPTQDTKRPAPSGDEPLDLSEPEDWGEASPFESRQAPRTKADLLNEIRQRTDRLT
GQQEAEQRQIDPWSAQPPQRRGHGR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1000
Plasmid name pBBKW-1
GenBank accession number NC_021875.1
Genome size 7716 bp
Coordinate of oriT  [Strand] 3922..3953 [+]; 7606..7637 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Bifidobacterium kashiwanohense JCM 15439 [1150460]
Reference
[1] Takahata M et al (2014) Complete sequence analysis of two cryptic plasmids from Bifidobacterium kashiwanohense JCM 15439 (type strain) isolated from healthy infant feces. Anim Sci J. 85(2):158-63. [PMID:23865717]