oriTDB

The information of the oriT region

oriTDB accession number	100539
Name	oriT1_pBBKW-1
Sequence Completeness	core
oriT length	32 nt
oriT sequence	ATGCAACCTCCGGTTGCATGTAAGTGCGCCCT
IR (inverted repeat)	[1-8] [12-19] nt (ATGCAACC..GGTTGCAT)
Location of nic site	[28-29] nt
Conserved sequence flanking the nic site	TAAGTGCG\|CCCT
Note	predicted by the oriTfinder
	L:1-8;R:12-19;D:28-29

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

ATGCAACCTCCGGTTGCATGTAAGTGCGCCCT

Reference
N/A

I. Information of Relaxase

ID	610
Name	Mob_pBBKW-1
GenBank accession number	YP_008327316
Family	MOB_Q
Length	565 aa
UniProt ID	S6BIT5
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 8.90E-66, Aligned region: 17..254
Note	putative relaxase
Protein sequence [Download]	MAIYHLSVSNVSRASGSKATATLSYISGKRVHDERRGETYDYGRKERVLRVGTLLPEGAP AEYADPAVLFNAVELHETGRTARPAKKIVVALPREFTPRQRVQALEEYIRENLNADGYAA TYAIHEDREGNNPHAHILVANRQIDPATGGWARLKQRMEYVLDERGERVPLIDPETGRQK TDKRGRRQWKRTSVSLNPLDRKAKLKALRESWANTCNARLDETARIDHRSLEDQGSDLEP TIHEGYAARAIERAGGVSERCQTNREIRRSNSLLTAVRAELGRIFDRLGELFAAKIRQLR QRQARPEPAREPNWRYFEGDARRQLEADKADHTAAIRGKLMDARADVGNREEWWRSHGTE EHEAAKQIIDVARAASREARDANIFKRGRLLRNAEQVAGEQSEKLRGAVPWLEDTDIPAD WDQANRFRMRTTKAIHDHDMQPRTRLVAELERRLEQAERAESEKPSPEQVKALALRMAEQ RAQAENPTQDTKRPAPSGDEPLDLSEPEDWGEASPFESRQAPRTKADLLNEIRQRTDRLT GQQEAEQRQIDPWSAQPPQRRGHGR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1000
Plasmid name	pBBKW-1
GenBank accession number	NC_021875.1
Genome size	7716 bp
Coordinate of oriT [Strand]	3922..3953 [+]; 7606..7637 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Bifidobacterium kashiwanohense JCM 15439 [1150460]

Reference

[1] Takahata M et al (2014) Complete sequence analysis of two cryptic plasmids from Bifidobacterium kashiwanohense JCM 15439 (type strain) isolated from healthy infant feces. Anim Sci J. 85(2):158-63. [PMID:23865717]