oriTDB

The information of the oriT region

oriTDB accession number	100538
Name	oriT_pSEEH1578_03
Sequence Completeness	intact
oriT length	186 nt
oriT sequence	GGTTTTAGCAGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTAT ACTGGCTCAATATGTTACGGCGGAGTAACACGTAATGAAAGTGCTTCATGTGGCAGGAGA AAAATGCAGCTGCAGGGCGTACAGGAGAAAATGGAGGTTACGGTGATATGACGCCTCCTC GCTCAC
IR (inverted repeat)	[14-21] [25-32] nt (TTCGGGGC..GCCCTGAA)
Location of nic site	[65-66] nt
Conserved sequence flanking the nic site	CTGG\|CTCA
Note	predicted by the oriTfinder
	L:14-21;R:25-32;D:65-66

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGTTTTAGCAGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTAT

61

ACTGGCTCAATATGTTACGGCGGAGTAACACGTAATGAAAGTGCTTCATGTGGCAGGAGA

121

AAAATGCAGCTGCAGGGCGTACAGGAGAAAATGGAGGTTACGGTGATATGACGCCTCCTC

181

GCTCAC

Reference
N/A

I. Information of Relaxase

ID	609
Name	Nuclease_pSEEH1578_03
GenBank accession number	WP_000635829
Family	MOB_P
Length	499 aa
UniProt ID	_
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.10E-16, Aligned region: 41..275
Note	putative relaxase
Protein sequence [Download]	MIVKFHPRGRGGGAGPVDYLLGKDRQRDGATVLQGKPEEVRELIDASPYAKKYTSGVLSF AEKDLPPGQREKLMASFERVLMPGLDKDQYSVLWVEHQDKGRLELNFLIPNMELLTGRRL QPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSALPKTKQEAAQAITRGLLA LASSGGLKTRQDVTEALESAGFEVVRTTKSSISIADPDGGRNIRLKGAIYEQSFNAGEGL RAEIESAAAEYRRDAESRIQRAREVCQSGTERKREENQRRHPRPRPEAVLSHEPAHERHG AYGQPDVADHRPGVYTADSIGRKHSMVAGTADTRQLHDHPGAEEHAGHAVREEQRRADLD LRRAETPLREGEPGSGTVRRGFELDDTGGEIAHDGAGKTVAERIRAATAGLLEKAGRVGE RLRGMADDVWSYATGERGAERARHGLEQAGAEFERAAAPVVTRLNVIEAHREQERAAQHQ KALELERSQRQRTYNGPSL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	999
Plasmid name	pSEEH1578_03
GenBank accession number	NC_021869.1
Genome size	4773 bp
Coordinate of oriT [Strand]	2905..3090 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Salmonella enterica subsp. enterica serovar Heidelberg str. 41578 [1124936]

Reference
N/A