oriTDB

The information of the oriT region

oriTDB accession number	100537
Name	oriT_pCFSAN001921-2
Sequence Completeness	intact
oriT length	181 nt
oriT sequence	TTAGCGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTG GCTTAATATAGTAAATCGAAGTGGGAATTCAGGGAAGTGCTTCATGTGACAGAGGAAAAA TGCGGCTGTGGGGCGTAAGTGCAGAATGTAGGTAAAAGTGAAATGACGCCTCCTCGCTCA C
IR (inverted repeat)	[10-17] [21-28] nt (TTCGGGGC..GCCCTGAA)
Location of nic site	[61-62] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:10-17;R:21-28;D:61-62

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TTAGCGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTG

61

GCTTAATATAGTAAATCGAAGTGGGAATTCAGGGAAGTGCTTCATGTGACAGAGGAAAAA

121

TGCGGCTGTGGGGCGTAAGTGCAGAATGTAGGTAAAAGTGAAATGACGCCTCCTCGCTCA

181

C

Reference
N/A

I. Information of Relaxase

ID	608
Name	Putative relaxase of pCFSAN001921-2
GenBank accession number	WP_020833678
Family	MOB_P
Length	499 aa
UniProt ID	_
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.90E-17, Aligned region: 42..273
Note	putative relaxase
Protein sequence [Download]	MIVKFHPRGRGGGAGPVDYLLGKDRHRDGASVLQGKPEEVRELIDASPYAKKYTSGVLSF AEQDLPPGQREKLMASFERVLMPGLDKDQYSVLWVEHNDKGRLELNFLIPNTELLTGRRL QPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALATPSALPETKQEAAQTITRGLLA LASSGELKTRQDVTEALENAGFEVVRTTKSSISIADPDGGRNIRLKGAIYEQSFNAGEGL RAEIESAAADYRRDAESRIQRAREVCQSGTERKREENQRRHPRPRPEAVLSHEPAHERDA AHGQPDVADHRAGLRAADRLERGHSVVAGAADTRELHDHPGAEEHAGHAVREEQRCAAHD LRRAETPLREGEPGSGRVRRGFELDDTGGEIAHDGAGKTAAERIRAATAGLLEKAGRVGE RLRGMADDVWSYATGERGAERARHGLEQAGAEFERAASPVVVRLDGIEAHREHERAVQHQ KTLELERSQRQRTYNGPSL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	998
Plasmid name	pCFSAN001921-2
GenBank accession number	NC_021843.1
Genome size	4675 bp
Coordinate of oriT [Strand]	2975..3155 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Salmonella enterica subsp. enterica serovar Typhimurium var. 5- str. CFSAN001921 [1271862]

Reference

[1] Hoffmann M et al (2013) Complete Genome Sequence of a Multidrug-Resistant Salmonella enterica Serovar Typhimurium var 5- Strain Isolated from Chicken Breast. Genome Announc. 1(6). [PMID:24356834]