oriTDB

The information of the oriT region

oriTDB accession number	100531
Name	oriT_pDB2011
Sequence Completeness	core
oriT length	39 nt
oriT sequence	CTTTACGAATGTAAAGTATAGTGTGTTATACTTTACATG
IR (inverted repeat)	[1-6] [11-16] nt (CTTTAC..GTAAAG)
Location of nic site	[25-26] nt
Conserved sequence flanking the nic site	GTG\|TTA
Note	predicted by the oriTfinder
	L:1-6;R:11-16;D:25-26

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CTTTACGAATGTAAAGTATAGTGTGTTATACTTTACATG

Reference
N/A

I. Information of Relaxase

ID	602
Name	Mob_pDB2011
GenBank accession number	YP_008119849
Family	MOB_V
Length	408 aa
UniProt ID	R9SAC3
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 5.80E-71, Aligned region: 1..193
Note	putative relaxase
Protein sequence [Download]	MSMIAARMQKLKAGNLVGIGNHNQRKTKNHSNPDIDTSLSKLNYDLVDRTQNYKTDIENF INENKSTTRAVRKDAVLVNEWIISSDKDFFDNLTESEIENFFERSKDYFAEKFGEKNIRY ATVHLDESTPHMHMGIVPFDKDNKLSAKRVFNRQALRDVQEELPKYLQDFQFEIARGQKG SERKNLTVPEFKKLKEEELEIKKELQIKKDELIAYTKENQIDKKLDITPIKEMEDIEIET DEKTLFGKNKTEIVRQWTGNIILSENDYLKLNKEIKKGKKTEGRLAAILETDVYQENKEL KNELKDQIDKNDKDIDDYNDLVKRYNNLYEENTSLKSQIGDLKEEIKLIYQSTKRFLKDR ISDFKAFKEVFKELADNISNISREKGLDSSFKKEFDRENKKKQTRGIR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	992
Plasmid name	pDB2011
GenBank accession number	NC_021513.1
Genome size	7641 bp
Coordinate of oriT [Strand]	7563..7601 [+]
Drug resistance	spectinomycin resistanc; trimethoprim resistance; resistance to macrolides
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Listeria innocua TTS-2011 [1642]

Reference

[1] Bertsch D et al (2013) pDB2011, a 76 kb multidrug resistance plasmid from Listeria innocua replicating in Gram-positive and Gram-negative hosts. Plasmid. 70(2):284-7. [PMID:23774482]