oriTDB

The information of the oriT region

oriTDB accession number	100527
Name	oriT_pSA8589
Sequence Completeness	core
oriT length	41 nt
oriT sequence	CACTTTATGAATATAAAGTATAGTGTGTTATACTTTACATG
IR (inverted repeat)	[2-8] [13-19] nt (ACTTTAT..ATAAAGT)
Location of nic site	[27-28] nt
Conserved sequence flanking the nic site	GTG\|TTA
Note	predicted by the oriTfinder
	L:2-8;R:13-19;D:27-28

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CACTTTATGAATATAAAGTATAGTGTGTTATACTTTACATG

Reference
N/A

I. Information of Relaxase

ID	598
Name	Mob_pSA8589
GenBank accession number	YP_007988854
Family	MOB_V
Length	408 aa
UniProt ID	R4NGV8
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 2.30E-71, Aligned region: 1..193
Note	putative relaxase
Protein sequence [Download]	MSMIVARMQKMKAENLVGIGNHNQRKTKNHSNPDIDTSLSKLNYDLVDRTQNYKTDIENF INENKSTTRAVRKDAVLVNEWIISSDKDFFDNLTESEIENFFERSKDYFAEKFGEKNIRY ATVHLDESTPHMHMGIVPFDKDNKLSAKRVFNRQALRDVQEELPKYLQDFQFEIARGQKG SERKNLTVPEFKKLKEEELEIKKELQIKKDELIAYTKENQIDKKLDITPIKEMEDIEIET DEKTLFGKNKTEIVRQWTGNIILSENDYLKLNKEIKKGKKTEGRLAAILETDVYQENKEL KNELKDQIDKNDKDIDDYNDLVKRYNNLYEENTSLKSQIGDLKEEIKLIYQSTKRFLKDR ISDFKAFKEVFKELADNISNISREKGLDSSFKKEFDRENKKKQTRGIR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	988
Plasmid name	pSA8589
GenBank accession number	NC_021230.1
Genome size	6962 bp
Coordinate of oriT [Strand]	3535..3575 [+]
Drug resistance	gene responsible for resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins and streptogramin A
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Staphylococcus aureus 1 [1280]

Reference

[1] Mendes RE et al (2013) Dissemination of a pSCFS3-like cfr-carrying plasmid in Staphylococcus aureus and Staphylococcus epidermidis clinical isolates recovered from hospitals in Ohio. Antimicrob Agents Chemother. 57(7):2923-8. [PMID:23571552]