oriTDB

The information of the oriT region

oriTDB accession number	100526
Name	oriT_pSTU288-3
Sequence Completeness	intact
oriT length	174 nt
oriT sequence	GGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTAC TATGTTGGCACGGATGCGAGTGTCAGTGAAGTGCTTCATGTGGCAGGAGAAAAATGCAGC AGCGGAGCGTCAGCAGAATATGTGATACAGGATATATTCCGCTTCCTCGCTCAC
IR (inverted repeat)	[2-11] [15-24] nt (GTTTCGGGGC..GCCCTGAACC)
Location of nic site	[55-56] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:2-11;R:15-24;D:55-56

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	597
Name	Nuclease_pSTU288-3
GenBank accession number	WP_015589618
Family	MOB_P
Length	499 aa
UniProt ID	_
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 3.30E-16, Aligned region: 49..247
Note	putative relaxase
Protein sequence [Download]	MIVKFHPRGRGGGAGPVDYLLGKDRQREGATVLQGKPEEVRELIDASPYVKKYTSGVLSF AEADLPSGQREKLMASFERVLMPGLDKDQYSILWVEHSDKGRLELNFLIPNTELLTGRRL QPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSALPKAKQEAAEAITRGLLA LASSGELKTRQDVTEALESAGFEVVRTTKSSISIADPDGGRNIRLKGAIYEQSFNAGEGL RAEIESAAAEYRRDAESRIQRAREICKSGTERKREENQRRHPRPRPETVLSHEPAHERHG AHGQPDVADHRPGLRAADRLERGHSVVAGAADTRQLHDHPGAEEHAGHAVREEQRRAALD LRRAETPLREGEPGSGTVRRGFELDDTGGEIAHDGAGKTVAERIRAATAGLLEKAGRVGE RLRGMADDVWSYATGERSAERARHGLEQAGAEFERAAAPVVERLNAVELHREQERVVQHQ KALELERSQRQRTYNSPSL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	987
Plasmid name	pSTU288-3
GenBank accession number	NC_021157.1
Genome size	4675 bp
Coordinate of oriT [Strand]	705..878 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Salmonella enterica subsp. enterica serovar Typhimurium str. U288 [1171376]

Reference

[1] Hooton SP et al (2014) The complete plasmid sequences of Salmonella enterica serovar Typhimurium U288. Plasmid. 76:32-9. [PMID:25175817]