 oriTDB accession number | 100524 |
| Name | oriT_pSTU288-1  |
Sequence Completeness  | intact |
| oriT length | 110 nt |
| oriT sequence | TCACTTCAGGCTCCTTACGGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATA GCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTCAGG |
| IR (inverted repeat) | IR1: [24-31] [35-42] nt (GTCGGGGC..GCCCTGAC) IR2: [49-65] [72-88] nt (GCAATTGTAATAGCGTC..GACGGTATTACAATTGC) |
| Location of nic site | [96-97] nt |
| Conserved sequence flanking the nic site | CATCCTG|T |
| Note | predicted by the oriTfinder |
Visualization of oriT structure Reference| ID | 595 |
| Name | TraI_pSTU288-1 |
| GenBank accession number | WP_000986921 |
| Family | MOBF |
| Length | 1752 aa |
| UniProt ID | _ |
| PDB ID | _ |
| Pfam | TrwC [PF08751.10], Evalue: 1.30E-76, Aligned region: 10..284 TraI [PF07057.10], Evalue: 6.80E-59, Aligned region: 1430..1553 AAA_30 [PF13604.5], Evalue: 1.10E-34, Aligned region: 967..1163 AAA_19 [PF13245.5], Evalue: 5.10E-09, Aligned region: 972..1107 |
| Note | putative relaxase |
| Protein sequence [Download] | MMSIAQVRSAGSAAGYYSDRDNYYVLGSMEERWAGKGAEQLGLQGTVDKEVFTRVLEGRL PDGADLSRQQDGGNKHRPGYDLTFSAPKSVSLMAMLAGDKRLTEAHNQAVDIAVRQVEAL ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHAVVVNVTQHDGEWKTLSSDKV GKTGFIENVYANQIAFGKIYRAVLKEKVEALGYETEVVGKHGMWEMPGVPVEAFSSRSQA IREAVGEDASLKSRDVAALDTRKSKQHVDPEVKMAEWMQTLKDTGFDISAYRESADRRAE IQAAQPVPSQEQPDIQQAVTQAIAGLSDRKVQFTYTDVLARTVGMLPPEAGVIEKARAGI DEAISREQLIPLDREKGLFTSGIHVLDELSVRALSSDIMKQNRVTVHPEKSVPRTGSYSD AVSVLAQDRPSLAIISGQGGAAGQRERVAELTMMAREQGREVQIIVADRRSQTNLKQDER LSGELITGRRQLQEGMLFSPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRT GTGSALMAMKEAGVNSYRWQGRQQTPATVISEPDRNVRYARLAGDFVAAVKAGEESVAQV SGMREQAILAGMIRSELKTQGILGQQDTMMTALSPVWLDSRNRYLRDMYREGMVMEQWNP EKRSHDRYVIDRVTAQSHSLTLRDAQGETQMVRISALDSSWSLFRPEKMPVADGERLMVT GKIPGLRVSVGDRLQVSAVNDGVMTVTVPGRAEPASLPVGDSPFTALKLENGWVETPGHS VSDSAKVFASVTQMAMDNATLNSLARSGRDVRLYSSLDETRTAEKLSRHPSFTVVSEQIK ARAGEVSLETAISRQKAGLHTPAQQAIHLALPVVESKNLAFSQVDLLTEAKSFAAEGTGF ADLGREIDAQIKRGDLLYVDVAKGYGTDLLISRASYDAEKSILHHILEGKEAVTPLMERV PGELMETLTSGQRAATRMILETKDRFTVVQGYAGVGKTTQFRAVMSAVNLLPESERPRVV GLGPTHRAVGEMRSAGVEAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMA RAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELRDAVY SLINRDVNKALSGLENVKPVQVPRLKGAWAPENSVTEFSRLQERELAKAAQEAEKKGEAF PDVPVTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIQDALAKPGEQQVTVPVL TTANIRDGELRRLSTWEAHPGALALVDNVYHRIAGISKEDGLITLEDKAGNTRLISPREA AAEGVTLYNPETIRVGAGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGKQTRVVR PGQDRAEQHIDLAYAITAHSAQGASETFAIALEGTEGGRKQMAGFESAYVALSRMKQHVQ VYTDDRQGWVKAINSAEQKGTAHDVLEPKSEREMMNAERLFSTARELRDVAAGRAVLRNA GLAQGDSRARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGAGLRGFTGEGRVK GSEEAQFVALQGSRNGESLLAGSMQDGVRIARENPDSGVVVRIVGDGRPWNPVAITGGRV WGDIPDSNVQPGAGNGEPVTAEILAQRQAEEAVRRETERRADEIVRKMAEDKSDLPEEKT AQAVREIAGQEQDRMTPPEREPPLPESVLREPQRERETIREVARENRGRERLQQMEQEMV RDLQKEKTPGGD |
Reference| ID | 985 |
| Plasmid name | pSTU288-1 |
| GenBank accession number | NC_021155.1 |
| Incompatibility group | IncFIB(S) |
| Genome size | 148711 bp |
| Coordinate of oriT [Strand] | 143487..143596 [+] |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | Salmonella plasmid virulence protein |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Salmonella enterica subsp. enterica serovar Typhimurium str. U288 [1171376] |
Reference| [1] Hooton SP et al (2014) The complete plasmid sequences of Salmonella enterica serovar Typhimurium U288. Plasmid. 76:32-9. [PMID:25175817] |