oriTDB

The information of the oriT region

oriTDB accession number	100515
Name	oriT_pN-Cit
Sequence Completeness	intact
oriT length	355 nt
oriT sequence	ACTCCGATACGTTGCCCTCCGAACGGTATTCAAGGTCGATTTTTTGCGCTTCGGTCACTC TTAAACTGATAGATGGCATAGGTTTCCTTTGTGTAATACCGATGTAATACATACAAATCT AGCATAGATGCGGCTTAATTCCACATATGTAATACGCTGTGTATTACATATTAAAACACA AATTAGAATAATTTGTTTTGTTTTCAAGCATTTACGATGAAAATCGTAATTGCGTATGGT GTATAGCCATTAAGGGATACCATAACACGCCTTTTTTAAGGGAGAAACAGCTCTTACACA CGCATTAAACGCTCAAAAAGCGTTCACATTCACCACTTTCATGCTTGCATGAAAG
IR (inverted repeat)	IR1: [145-155] [161-171] nt (ATATGTAATAC..GTATTACATAT) IR2: [208-218] [223-233] nt (GCATTTACGAT..ATCGTAATTGC) IR3: [336-344] [347-355] nt (CTTTCATGC..GCATGAAAG)
Location of nic site	[242-243] nt
Conserved sequence flanking the nic site	GGTGT\|ATAGC
Note	predicted by the oriTfinder
	L:145-155;R:161-171;L:208-218;R:223-233;L:336-344;R:347-355;D:242-243

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

ACTCCGATACGTTGCCCTCCGAACGGTATTCAAGGTCGATTTTTTGCGCTTCGGTCACTC

61

TTAAACTGATAGATGGCATAGGTTTCCTTTGTGTAATACCGATGTAATACATACAAATCT

121

AGCATAGATGCGGCTTAATTCCACATATGTAATACGCTGTGTATTACATATTAAAACACA

181

AATTAGAATAATTTGTTTTGTTTTCAAGCATTTACGATGAAAATCGTAATTGCGTATGGT

241

GTATAGCCATTAAGGGATACCATAACACGCCTTTTTTAAGGGAGAAACAGCTCTTACACA

301

CGCATTAAACGCTCAAAAAGCGTTCACATTCACCACTTTCATGCTTGCATGAAAG

Reference
N/A

I. Information of Relaxase

ID	586
Name	TraI_pN-Cit
GenBank accession number	YP_007354951
Family	MOB_F
Length	967 aa
UniProt ID	L7SYL3
PDB ID	_
Pfam	TrwC [PF08751.10], Evalue: 5.70E-80, Aligned region: 12..288 AAA_30 [PF13604.5], Evalue: 2.90E-40, Aligned region: 475..659 Viral_helicase1 [PF01443.17], Evalue: 1.10E-07, Aligned region: 495..655 Viral_helicase1 [PF01443.17], Evalue: 2.30E-06, Aligned region: 780..897 UvrD_C_2 [PF13538.5], Evalue: 2.00E-09, Aligned region: 849..896
Note	putative relaxase
Protein sequence [Download]	MLNITTITRQRVDKVVDYYADGADDYYAKDGNAMQWQGAGAEELGLTGEVEQKRFAKLLD GKVTDDISVMRKTANSESKERLGYDLTFSAPKGVTLQALVNGDKRIIEAHDRAVTKAIEE AERLALGRFTVNKKTHVENTNKLIVGKFRHETSRELDPDLHTHAFVMNLTKTSDGKWRSL TNDGIVNSLTLLGNVYKSELARELELAGYNLRYDRNGTFDLAHFSQEQIAQFSQRSQQIE AELAKNGLSRETASHEQKNAAALKTRKSKGEVDRDVLYEGWKNRAKELQIDFDSREWKGA ANDDIARNTQPAALEKPIEYQADKVMTFAIRSLTERQSVITERELMDVAMKHGYGRLTLD DIRAARERAITSGNLIKEEATYAAATTNKKQQNVAMTREQWVTELVKAGRNKDEARKLVD KGITNGRLVQQKPRFTTQLAQQRERNILKMEREGRGKIQTPYTREFSEGWLASRTLKPEQ LKAVMGIIHTPNQFISVHGFAGTGKSYMTKSAADFLKEQGVHVTSLAPYGSQVKALQAEG LESRTLQSFLRASDKKIGPGSVVFIDEAGVIPARQMEETMRVIRDAGARAVFLGDTKQTK AVEAGKPFEQLIKAGMETAYMKDIQRQKDPELLKAALNAAEGKIKASLTHVTSIVEEKNH DQRYRRIVGDYVAMPPTDRANALIITGTNDSRKKINAYIQAELGLKGQGINYPLLNRLDT TQAQRQHSKYYEKGAVIIPERDYSNGLKRGAVYTVLDTGPGNKLTVSDGSGDTIAFSPAR FSKLSVYSVEKTELAVGDQVRITRNDAHLDLANGDRFKVKGVQSGEVLLENEKGRLVKID ANKPMYLGLAYASTVHSAQGLTCDKVFINMDTRSLTTAKDVYYVAVTRAKHEAVIYTDDE KKLDKAASREGFKTAALELEQLKRYAKEQQHDAREKGRDKTEPTQEKDNAKRKGHDKNND ERAFTSL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	976
Plasmid name	pN-Cit
GenBank accession number	NC_020122.1
Genome size	34826 bp
Coordinate of oriT [Strand]	17167..17521 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Citrobacter freundii CFSTE [546]

Reference

[1] Villa L et al (2013) Complete sequence of the IncT-type plasmid pT-OXA-181 carrying the blaOXA-181 carbapenemase gene from Citrobacter freundii. Antimicrob Agents Chemother. 57(4):1965-7. [PMID:23357767]