oriTDB
The information of the oriT region
oriTDB accession number 100515
Name oriT_pN-Cit  insolico
Sequence Completeness intact
oriT length 355 nt
oriT sequence ACTCCGATACGTTGCCCTCCGAACGGTATTCAAGGTCGATTTTTTGCGCTTCGGTCACTC TTAAACTGATAGATGGCATAGGTTTCCTTTGTGTAATACCGATGTAATACATACAAATCT AGCATAGATGCGGCTTAATTCCACATATGTAATACGCTGTGTATTACATATTAAAACACA AATTAGAATAATTTGTTTTGTTTTCAAGCATTTACGATGAAAATCGTAATTGCGTATGGT GTATAGCCATTAAGGGATACCATAACACGCCTTTTTTAAGGGAGAAACAGCTCTTACACA CGCATTAAACGCTCAAAAAGCGTTCACATTCACCACTTTCATGCTTGCATGAAAG
IR (inverted repeat)IR1: [145-155] [161-171] nt  (ATATGTAATAC..GTATTACATAT)
IR2: [208-218] [223-233] nt  (GCATTTACGAT..ATCGTAATTGC)
IR3: [336-344] [347-355] nt  (CTTTCATGC..GCATGAAAG)
Location of nic site [242-243] nt
Conserved sequence flanking the
  nic site 		GGTGT|ATAGC
Note predicted by the oriTfinder
L:145-155;R:161-171;L:208-218;R:223-233;L:336-344;R:347-355;D:242-243
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 586
Name TraI_pN-Cit insolico
GenBank accession number YP_007354951
Family MOBF
Length 967 aa
UniProt ID L7SYL3
PDB ID _
Pfam TrwC [PF08751.10], Evalue: 5.70E-80, Aligned region: 12..288
AAA_30 [PF13604.5], Evalue: 2.90E-40, Aligned region: 475..659
Viral_helicase1 [PF01443.17], Evalue: 1.10E-07, Aligned region: 495..655
Viral_helicase1 [PF01443.17], Evalue: 2.30E-06, Aligned region: 780..897
UvrD_C_2 [PF13538.5], Evalue: 2.00E-09, Aligned region: 849..896
Note putative relaxase
Protein sequence [Download] MLNITTITRQRVDKVVDYYADGADDYYAKDGNAMQWQGAGAEELGLTGEVEQKRFAKLLD
GKVTDDISVMRKTANSESKERLGYDLTFSAPKGVTLQALVNGDKRIIEAHDRAVTKAIEE
AERLALGRFTVNKKTHVENTNKLIVGKFRHETSRELDPDLHTHAFVMNLTKTSDGKWRSL
TNDGIVNSLTLLGNVYKSELARELELAGYNLRYDRNGTFDLAHFSQEQIAQFSQRSQQIE
AELAKNGLSRETASHEQKNAAALKTRKSKGEVDRDVLYEGWKNRAKELQIDFDSREWKGA
ANDDIARNTQPAALEKPIEYQADKVMTFAIRSLTERQSVITERELMDVAMKHGYGRLTLD
DIRAARERAITSGNLIKEEATYAAATTNKKQQNVAMTREQWVTELVKAGRNKDEARKLVD
KGITNGRLVQQKPRFTTQLAQQRERNILKMEREGRGKIQTPYTREFSEGWLASRTLKPEQ
LKAVMGIIHTPNQFISVHGFAGTGKSYMTKSAADFLKEQGVHVTSLAPYGSQVKALQAEG
LESRTLQSFLRASDKKIGPGSVVFIDEAGVIPARQMEETMRVIRDAGARAVFLGDTKQTK
AVEAGKPFEQLIKAGMETAYMKDIQRQKDPELLKAALNAAEGKIKASLTHVTSIVEEKNH
DQRYRRIVGDYVAMPPTDRANALIITGTNDSRKKINAYIQAELGLKGQGINYPLLNRLDT
TQAQRQHSKYYEKGAVIIPERDYSNGLKRGAVYTVLDTGPGNKLTVSDGSGDTIAFSPAR
FSKLSVYSVEKTELAVGDQVRITRNDAHLDLANGDRFKVKGVQSGEVLLENEKGRLVKID
ANKPMYLGLAYASTVHSAQGLTCDKVFINMDTRSLTTAKDVYYVAVTRAKHEAVIYTDDE
KKLDKAASREGFKTAALELEQLKRYAKEQQHDAREKGRDKTEPTQEKDNAKRKGHDKNND
ERAFTSL
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 976
Plasmid name pN-Cit
GenBank accession number NC_020122.1
Genome size 34826 bp
Coordinate of oriT  [Strand] 17167..17521 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Citrobacter freundii CFSTE [546]
Reference
[1] Villa L et al (2013) Complete sequence of the IncT-type plasmid pT-OXA-181 carrying the blaOXA-181 carbapenemase gene from Citrobacter freundii. Antimicrob Agents Chemother. 57(4):1965-7. [PMID:23357767]

Microbial Bioinformatics Group in MML, SJTU