oriTDB

The information of the oriT region

oriTDB accession number	100514
Name	oriT_pXF-RIV5
Sequence Completeness	intact
oriT length	103 nt
oriT sequence	AGCCTCGCAGAGCAGGATTCCCGTTGAGTACCGAAGGTGCGAATAAGGGAAAGTGAAGAA GGAACACCGGATTTCCGGTGGGCCTACTTCACATATCCTGCCC
IR (inverted repeat)	[53-70] [75-92] nt (GTGAAGAAGGAACACCGG..CCGGTGGGCCTACTTCAC)
Location of nic site	[100-101] nt
Conserved sequence flanking the nic site	TATCCTG\|C
Note	predicted by the oriTfinder
	L:53-70;R:75-92;D:100-101

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AGCCTCGCAGAGCAGGATTCCCGTTGAGTACCGAAGGTGCGAATAAGGGAAAGTGAAGAA

61

GGAACACCGGATTTCCGGTGGGCCTACTTCACATATCCTGCCC

Reference
N/A

I. Information of Relaxase

ID	585
Name	TraI_pXF-RIV5
GenBank accession number	YP_007354920
Family	MOB_P
Length	830 aa
UniProt ID	L7SYI0
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.60E-53, Aligned region: 12..252
Note	putative relaxase
Protein sequence [Download]	MIAKHVPMRVVKKSDFRELVKYLSNQQGKQERVGCVTVTNCFQNNVLDAALEVQATQALN TRSEADKTYHLLISFREGENPAPEILEVIESRVCAALGYADYQRVSVVHHDTDNLHIHVA INKIHPKSYTIHTPYNDYKTLGEICKKLEREYGLEADNHTVRKTVGENRADDMERNAGVE SLLGWIKRECTDQIKQVQSWSELHAVMQRNGLEIRERGNGLVITNGVGRSVKASSVARDF SKAKLEARFGAFESFVKQAPSVSSVHARRIQAPLVEKVGRRPPPRSKGRTPSLSSVGVLH VNSGIRYEQRPIYVKQASTAELYAMYKLEQQDLGAVRNVAIARARTKRDRKIEATKRLGR IKRAAIKLMRGPGVNKKLLYALARKPLKEGVEKANTDYLKARDAAYATYHRRVWADWLQV QATQGNAEALAALRAREMRQWWSCNIFGGLQARRTGPVPGLKSDNITKAGTIIYRVGSTA IRDDGDLLNVSHGAGDYGVEAALRMAMYRYGERITVKGSDEFKKRVVQIAAAARLNISFD DEVLDKRRKQLVSDAAVIVKLTDAMLSDKTAQFTQAQASRVGAFQEDALSEYDAAISRSI NLANQEQINEQQRTRAKHYFVQRWSDRGGPNSRRDGHATRIRSGGGGSGKHGASGATVIS SAGGFNKPNVGGIGAKPPPASTHRLRNLHQLGVVQFSRGSEVLLPRHVSDCVGEQQTERN HRVRRDVHTAGITSSELMAVDKYIAERELKRVREFDIMKNRRYNEDDAGMVKFAGLRQVD GKSLVLLKRNDEVIVLAIDVATARRMKRFSLGDEVLLTKKGTLKTKGRNR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	975
Plasmid name	pXF-RIV5
GenBank accession number	NC_020121.1
Genome size	38297 bp
Coordinate of oriT [Strand]	26824..26926 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Xylella fastidiosa subsp. multiplex Riv5 [644357]

Reference

[1] Rogers EE et al (2012) A conjugative 38 kB plasmid is present in multiple subspecies of Xylella fastidiosa. PLoS One. 7(12):e52131. [PMID:23251694]