oriTDB

The information of the oriT region

oriTDB accession number	100510
Name	oriT_pB1023
Sequence Completeness	intact
oriT length	175 nt
oriT sequence	GGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTAC TATGTTGGCACGGATGAGGATGTCAGTGAAGTGCTTCATGTGGCAGGAGAAAAAAGGCGG CACCGGCGCGTCAGCAGAATATGTGATACAGGATATATTCCGCTTCCTCGCTCAC
IR (inverted repeat)	[2-11] [15-24] nt (GTTTCGGGGC..GCCCTGAACC)
Location of nic site	[55-56] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:2-11;R:15-24;D:55-56

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTAC

61

TATGTTGGCACGGATGAGGATGTCAGTGAAGTGCTTCATGTGGCAGGAGAAAAAAGGCGG

121

CACCGGCGCGTCAGCAGAATATGTGATACAGGATATATTCCGCTTCCTCGCTCAC

Reference
N/A

I. Information of Relaxase

ID	581
Name	MbeA_pB1023
GenBank accession number	YP_007316673
Family	MOB_P
Length	525 aa
UniProt ID	K9MQA8
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 4.50E-15, Aligned region: 66..264
Note	putative relaxase
Protein sequence [Download]	MPVWNGCGMPYWKKGRTMIVKFHPRGRGGGAGPVDYLLGKDRQREGASVLQGKPEEVREL IDASPYVKKYTSGVLSFAEADLPPGQREKLMASFERVLMPGLDKDQYSILWVEHADKGRL ELNFLIPNTELLTGKRLQPYYDRADRPRIDAWQTVVNGRLGLHDPNDPENRRALVTPSGL PETKQEAAETITRGLLMLASSGELKTRDDVTGALTAAGFEVVRTTKNSISIADPDGGRNI RLRGAIYEQSFNGLEGLRGEIESTAAAYRREAESRIQQARERCQIGTERKREENQRRHQR PRTGDERGDAVEPAERAANGRADVADHHHGVRAAVRQPERGAVVPGEPDSGEPGGNQAAE RYAVETAHADVGRDLSGEPQRTVSGAAGGDESRNGPDGGQQDAERGEAGTGVEQNDGTGK TIAERVRAATAGLLEKAGRVGERLRGMAEDVWSYATGERGAERARVGLEQAGAEFERAAA PVIDRLNEIEQQRYGQTTEKEPEKTADLVPERQRTLSHGDREYGD

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	971
Plasmid name	pB1023
GenBank accession number	NC_019986.1
Incompatibility group	IncN
Genome size	3510 bp
Coordinate of oriT [Strand]	238..412 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Enterobacter cloacae BB1092 [550]

Reference
N/A