oriTDB
The information of the oriT region
oriTDB accession number 100508
Name oriT_pEC159  insolico
Sequence Completeness intact
oriT length 83 nt
oriT sequence GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTA ACCATGCGGCATCAGTGCGGATT
IR (inverted repeat)[3-12] [16-25] nt  (GTGTCGGGGC..GCCCTGACCC)
Location of nic site [56-57] nt
Conserved sequence flanking the
  nic site 		CTGG|CTTA
Note predicted by the oriTfinder
L:3-12;R:16-25;D:56-57
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 579
Name MobA_pEC159 insolico
GenBank accession number YP_007316615
Family MOBP
Length 517 aa
UniProt ID K9KZT9
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 7.50E-20, Aligned region: 50..246
Note putative relaxase
Protein sequence [Download] MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF
AEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFIIPNMELQTGKRL
QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRILTLSDNLPETKQALAESVTRGIDA
LYHVGEIKGRQDVIQALTEAGLEVVRVTRSSISIADPNGGKNIRLKGAFYEQSFTDGRGV
REKAERESRIYRENAERRVQEARRVCQRGCDLKRDENQRRYSPVHSLDREIAGKTQGRGE
RGDDAAQEGRVKAGREYGHDVTGDSLSPVYREWRDALVSWREDTGEPGRNQEAGRDIAET
EREDMGRGVCAGREQEISGPSVREISRGDSLSGERVGTSEGVTQNDRAGNTFAERLRAAA
TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREL
EIREERLIQEREHALSLERERQLEIQERTLDGPSLGW
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 969
Plasmid name pEC159
GenBank accession number NC_019982.1
Incompatibility group ColRNAI
Genome size 6292 bp
Coordinate of oriT  [Strand] 833..915 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli [562]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU