oriTDB
The information of the oriT region
oriTDB accession number 100506
Name oriT_pCIS8  insolico
Sequence Completeness intact
oriT length 464 nt
oriT sequence TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAA AAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGAC AAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAA AGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAG CCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTA TGGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATT ACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGA GGCTATTTATGGATCAGAAAAATGTATCACAAAATCAAACAAAG
IR (inverted repeat)IR1: [139-148] [153-162] nt  (CCCATTTTAT..ATAAAATGGG)
IR2: [208-220] [224-236] nt  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
IR3: [242-256] [269-283] nt  (CACATTGTAATACAA..TTGTATTACAATGTG)
Location of nic site [291-292] nt
Conserved sequence flanking the
  nic site 		CTTG|CA
Note predicted by the oriTfinder
L:139-148;R:153-162;L:208-220;R:224-236;L:242-256;R:269-283;D:291-292
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 577
Name MobA_pCIS8 insolico
GenBank accession number WP_015081763
Family MOBP
Length 410 aa
UniProt ID _
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 5.20E-75, Aligned region: 9..280
Note putative relaxase
Protein sequence [Download] MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGI
DPFIAKEQFDVIRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLY
PNHQSAVYTHLDGKNHVLHNHIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWH
ILEPPKERQTETEKELIAKNEYSYMDDLRERINKSLQDVSVSSYETFKERLSDNGVILSE
RGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELEKRTKQNEFSAVEQREPAITPLE
RDTQQRESEIVSLEQAIEPRKSEALKRESKINRFIDTIKQLAGRVPELTQRVTRKLKQTK
EKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSL
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 967
Plasmid name pCIS8
GenBank accession number NC_019430.1
Genome size 80592 bp
Coordinate of oriT  [Strand] 71532..71995 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Lactococcus lactis subsp. cremoris UC509.9 [1111678]
Reference
[1] Ainsworth S et al (2013) Complete Genome of Lactococcus lactis subsp cremoris UC5099, Host for a Model Lactococcal P335 Bacteriophage. Genome Announc. 1(1). [PMID:23405300]

Microbial Bioinformatics Group in MML, SJTU