oriTDB

The information of the oriT region

oriTDB accession number	100500
Name	oriT_pSB13
Sequence Completeness	intact
oriT length	83 nt
oriT sequence	GGGTGTCGGGGTGAAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTGA ACCATGCGGCATCAGTGCGGATT
IR (inverted repeat)	[3-11] [17-25] nt (GTGTCGGGG..CCCTGACCC)
Location of nic site	[56-57] nt
Conserved sequence flanking the nic site	CTGG\|CTGA
Note	predicted by the oriTfinder
	L:3-11;R:17-25;D:56-57

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGGTGTCGGGGTGAAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTGA

61

ACCATGCGGCATCAGTGCGGATT

Reference
N/A

I. Information of Relaxase

ID	571
Name	MobA_pSB13
GenBank accession number	YP_006964840
Family	MOB_P
Length	517 aa
UniProt ID	I6M980
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 6.30E-20, Aligned region: 49..246
Note	putative relaxase
Protein sequence [Download]	MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF AEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELETGKRL QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLPDNLPETKQALAEGVTRGIDA LYHAGEIKGRQDVIQALTEVGLEVVRVTRSSISIADPNGGRNIRLKGAFYEQSFTDGRGV REKAERESRIYRDNAERRVQQARKICKQGCDLKRDENQRRYSPVHSFDRGITEKTPGRGE RGDDAAQEGRVKAGREYGHDVTGDGHFPVYREWRDALVSWREDTGEPGRNQDTGRNIAET EREDMGRGVCAGREKEISGHPAGEIGNGDSLSGEGLGTTDGVTQSDGAGKTFAERLRATA TGLYAAAERMGERLRGIAENVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREQ EIRDERLIQEREHELSLERERQPEIQERTLDGPSLGW

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	961
Plasmid name	pSB13
GenBank accession number	NC_019357.1
Genome size	5424 bp
Coordinate of oriT [Strand]	2556..2638 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Shigella boydii C13 [621]

Reference
N/A