oriTDB

The information of the oriT region

oriTDB accession number	100499
Name	oriT_pNE1280
Sequence Completeness	intact
oriT length	216 nt
oriT sequence	GATGGCATGGGATGATATCCAGTAGCTGAGAGGTTGTTAGCGGGTTTCGGGGCGCAACCC TGAACCAGTCACGTAGCGCTAGCAGAGTGTATAATGGCTTATTATGTTGGCATGAGTGCG ATAATCAGGAAAGCGCCTCATGTGGCAGGAGAAAAATGCTGCAGCAGAGCGTAAGCAGAA TATGCGATACAGAAGATATTCCGCTTCCTCGCTCAC
IR (inverted repeat)	[46-52] [58-64] nt (TTCGGGG..CCCTGAA)
Location of nic site	[97-98] nt
Conserved sequence flanking the nic site	ATGG\|CTTA
Note	predicted by the oriTfinder
	L:46-52;R:58-64;D:97-98

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GATGGCATGGGATGATATCCAGTAGCTGAGAGGTTGTTAGCGGGTTTCGGGGCGCAACCC

61

TGAACCAGTCACGTAGCGCTAGCAGAGTGTATAATGGCTTATTATGTTGGCATGAGTGCG

121

ATAATCAGGAAAGCGCCTCATGTGGCAGGAGAAAAATGCTGCAGCAGAGCGTAAGCAGAA

181

TATGCGATACAGAAGATATTCCGCTTCCTCGCTCAC

Reference
N/A

I. Information of Relaxase

ID	570
Name	MobA_pNE1280
GenBank accession number	YP_006964655
Family	MOB_P
Length	659 aa
UniProt ID	I6TGP3
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.70E-30, Aligned region: 13..334
Note	putative relaxase
Protein sequence [Download]	MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTR KQKVISTPVDVEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSW PDYEKPNDDQVFDSVKFTLASMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSS FTKDTLHQACRLLELKNGWSHSNGAYVVNDRQQIVRNPHSKKERGNWRSLDRINKMENKE GVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHKTFAGIGLRVEKAQGKKGYVI THEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTAYTPGAYRRDA NKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAAL SQMRGFAYSSRKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGT VIFERDGKPVAADRGHIVLTESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCN RIVDAAVNHNHPVAQGITFTDAAQQAYAQNEKHRLIREQNNSKNEMQLRSESDDKFNPK

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	960
Plasmid name	pNE1280
GenBank accession number	NC_019346.1
Incompatibility group	IncL/M(pMU407)
Genome size	66531 bp
Coordinate of oriT [Strand]	31539..31754 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Enterobacter cloacae 1623 [550]

Reference

[1] Bryant KA et al (2013) KPC-4 Is encoded within a truncated Tn4401 in an IncL/M plasmid, pNE1280, isolated from Enterobacter cloacae and Serratia marcescens. Antimicrob Agents Chemother. 57(1):37-41. [PMID:23070154]