 oriTDB accession number | 100499 |
| Name | oriT_pNE1280  |
Sequence Completeness  | intact |
| oriT length | 216 nt |
| oriT sequence | GATGGCATGGGATGATATCCAGTAGCTGAGAGGTTGTTAGCGGGTTTCGGGGCGCAACCC TGAACCAGTCACGTAGCGCTAGCAGAGTGTATAATGGCTTATTATGTTGGCATGAGTGCG ATAATCAGGAAAGCGCCTCATGTGGCAGGAGAAAAATGCTGCAGCAGAGCGTAAGCAGAA TATGCGATACAGAAGATATTCCGCTTCCTCGCTCAC |
| IR (inverted repeat) | [46-52] [58-64] nt (TTCGGGG..CCCTGAA) |
| Location of nic site | [97-98] nt |
| Conserved sequence flanking the nic site | ATGG|CTTA |
| Note | predicted by the oriTfinder |
Visualization of oriT structure Reference| ID | 570 |
| Name | MobA_pNE1280 |
| GenBank accession number | YP_006964655 |
| Family | MOBP |
| Length | 659 aa |
| UniProt ID | I6TGP3 |
| PDB ID | _ |
| Pfam | Relaxase [PF03432.13], Evalue: 1.70E-30, Aligned region: 13..334 |
| Note | putative relaxase |
| Protein sequence [Download] | MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTR KQKVISTPVDVEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSW PDYEKPNDDQVFDSVKFTLASMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSS FTKDTLHQACRLLELKNGWSHSNGAYVVNDRQQIVRNPHSKKERGNWRSLDRINKMENKE GVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHKTFAGIGLRVEKAQGKKGYVI THEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTAYTPGAYRRDA NKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAAL SQMRGFAYSSRKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGT VIFERDGKPVAADRGHIVLTESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCN RIVDAAVNHNHPVAQGITFTDAAQQAYAQNEKHRLIREQNNSKNEMQLRSESDDKFNPK |
Reference| ID | 960 |
| Plasmid name | pNE1280 |
| GenBank accession number | NC_019346.1 |
| Incompatibility group | IncL/M(pMU407) |
| Genome size | 66531 bp |
| Coordinate of oriT [Strand] | 31539..31754 [-] |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Enterobacter cloacae 1623 [550] |
Reference| [1] Bryant KA et al (2013) KPC-4 Is encoded within a truncated Tn4401 in an IncL/M plasmid, pNE1280, isolated from Enterobacter cloacae and Serratia marcescens. Antimicrob Agents Chemother. 57(1):37-41. [PMID:23070154] |