oriTDB
The information of the oriT region
oriTDB accession number 100497
Name oriT_p14-120  insolico
Sequence Completeness intact
oriT length 360 nt
oriT sequence GATTATCAAGAGTCACATGTGAATCGGCCATTTATTTTATTAGAGTCAACATGGATTCAC CAACTTCATACAGGCGAATCTGTTATACCGAAATTAAGGATTCGAATCCAGATTCGAATC CTTAATTTTGTCTTAATTTAGTGGTCTGATTCGTAGATGAATCTGGATTCAACTCCTTTA CGATTCACTGAAGATTTGCATGTGAATCTAGTATCTATATCGAACATAGAATAACGATAA AAATATTAACATAACATACTGATATAAAAGGATAATCATGCAAGATTAAATCTTGCGACG GGTGTGGGACTTTGATGTTAAGGAGCAGGCATACAGAAATATGCCAGCCAGGAAGAATTC
IR (inverted repeat)[280-287] [289-296] nt  (GCAAGATT..AATCTTGC)
Location of nic site [305-306] nt
Conserved sequence flanking the
  nic site 		GACGGGTGT|GG
Note predicted by the oriTfinder
L:280-287;R:289-296;D:305-306
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 568
Name TraD_p14-120 insolico
GenBank accession number YP_006964311
Family MOBF
Length 1767 aa
UniProt ID I3W357
PDB ID _
Pfam TrwC [PF08751.10], Evalue: 3.40E-72, Aligned region: 7..280
TraI [PF07057.10], Evalue: 3.50E-47, Aligned region: 1441..1562
AAA_30 [PF13604.5], Evalue: 4.00E-35, Aligned region: 970..1162
AAA_19 [PF13245.5], Evalue: 4.30E-10, Aligned region: 975..1110
Note putative relaxase
Protein sequence [Download] MLSISSIKGDAGYYSHEDNYYASGSLDSRWMGEGAEKLGLKGEVASADMDAVRQGRLPDG
SDLSRMVDGVNKHRSGYDLTFSAPKSVSVMALVGEDRRFIEAHNRAVAVVMQEVEQLVSA
RITQEGKTETVLTGSMVAALYNHDTSRDLDPQVHTHALVFNATFADDKWRSLASDTRMKT
GFSENLYATKIALGNLYRAALREDIESMGFETVAAGKHGLWELKDVPVDIFSSRSQSIRK
AAGPDASAKSRDIAALDTRQAKAWADPELLKADWRRRLTDEKFDIDNYISQAQTRAEPPG
PVVGGTEGIRSTGQPASASSAQISELDVQKAVSDTISALSDKKVQFSWSEMLAGTVSRLP
SAPGLFVQARAAIEASIEGQRLIPLDREKGIFTSDIHLLNELSVHQLARTAIQEQTVLVF
PERAQERDIPAGDAVSVLSQDKSPVAILSGRGGAQTLRERTEDVAMMARSQGREVMVIAA
DGRSGQFLSESPHLAGHVMLRSQMNADTLLPVQGTVIVDRAERLSLKETVLLQEKALSAG
AQLIFMDTENRQGTGNALSVLKEADIPQYRFYSTQLPEVRLVSEADKRSRYSQLAQDYVR
LSAEGRDVVAQVTGAREQQQLTEVIRDTRREAGELGREQVTLRVLEPVWLDSKTRHQRDN
YRPGMVMEQWDAEKKTMTRHTIDRVAEATNSLVLLGEDGTRLTMKVTQLDGSWSLYRSRT
LEVSEGDRVRALGRELKGAIKAKEQFTVAGLENGAVRLRSGDRELRLPTERAVKLTHDYV
EGTGAGTSASRTVLAAVGPRGLNKQALNALAQSGSDIRIYTPREGRQAARKVESVSAVRL
ASDQVRQLTGEANLDTAIQASRERLMSDAEQAVNLAIPRAQQGQVYLSELTLLAEAVKSG
QPLADVRTEIARQVDSGALIKLDSVAGAGNRVLVPRVAYEMEKTIIRHIAEGKDAVQPLM
ALTPASVLSGLTAGQREATRTVLENTDRFMAIQGYAGVGKTTQFRAVMGALNTLSESVRP
QVIGLGPTHRAVHEMREAGVEARTLASFLSETRQAIQAGETPDFRNVLFLTDESSMVGNR
DMAELYQLVAAGGGRMVSSGDTAQLQAVSTGQPFRLVQQRSAIDTVVMQEIVRQTPALRP
AIESIIAGQVEISLRQVDDVTPQQVPRQPGAWVPDNSVMEIRAPKKEQDQVQDTPVAGEQ
TLTPEQLALVRTDIIEAIRDDWMGRTSEAQQQTLVVAELNADRHAINAAIHAARHEKGDT
GAEERTFTVLEPLRVPDNALRAAETFAEYTGAVAMMNERYWTVAEVDTQDAVVTLRNADG
ESVLISPQQNTAQDISLFTPRELTISQGDRVRFTRSDTDRGYVANSLWEVAGFTDGGAIR
FRQGEQEKIVDPQAMTEDRHIDLAYALTVYGVQGASERFAIALTGTEGGRKRMASLESTY
VTLSRAKEHVQVYTDNLEDWQQQVRQSDGGKTAHDLLHEKSDRESDTGNRLLATALRLDK
TALGRRVLAENGLEGETMARFIAAGKKYPSPYVALPAWTRHGKAAGALLTEIRIEDDGMR
VMLSDESRLRGGDDAQFAGLQASRNGQTLIADDAQTALRLAQENPESGVVIRLHGEERLL
NAARLTGGRITEPDEVERTVRSVAEAESAAKTEDPITLPPDEQQKLAEAQEKAARELAEQ
ARQELLPEMPGEESSRPEPLLSADEERRLRDGTARGERELDEAIQEAVADGRGIRQQVRE
QMLRTEREWVVNVPEKDIELEKTLGGD
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 958
Plasmid name p14-120
GenBank accession number NC_019342.1
Incompatibility group IncFII(p14)
Genome size 130175 bp
Coordinate of oriT  [Strand] 65932..66291 [+]
Drug resistance _
Heavy-metal resistance insolico Cobalt-zinc-cadmium resistance
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Salmonella sp. 14 [1179812]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU