oriTDB

The information of the oriT region

oriTDB accession number	100492
Name	oriT_pUR3937
Sequence Completeness	intact
oriT length	204 nt
oriT sequence	TGGCTAGCAATTAGCCACTCTTTTTTGCGTAAGCAAAAAACATAAGGGGCTTGGGGATAA TCCCCAACAAGCTGGCGCGTCTGCCACGTTAGTGGCTAGCAAAGCCAATGCTTGCCAAAC CACTTAACGACTTGATAAATTTTGCTTTATCTTATAAAGTAAAATTTAAGAAAGGCGTTA TCTTTCGTAGAAAGCGTGGTGGGA
IR (inverted repeat)	IR1: [51-58] [60-67] nt (TTGGGGAT..ATCCCCAA) IR2: [93-97] [103-107] nt (TGGCT..AGCCA)
Location of nic site	[114-115] nt
Conserved sequence flanking the nic site	TGCTTG\|CCA
Note	predicted by the oriTfinder
	L:51-58;R:60-67;L:93-97;R:103-107;D:114-115

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TGGCTAGCAATTAGCCACTCTTTTTTGCGTAAGCAAAAAACATAAGGGGCTTGGGGATAA

61

TCCCCAACAAGCTGGCGCGTCTGCCACGTTAGTGGCTAGCAAAGCCAATGCTTGCCAAAC

121

CACTTAACGACTTGATAAATTTTGCTTTATCTTATAAAGTAAAATTTAAGAAAGGCGTTA

181

TCTTTCGTAGAAAGCGTGGTGGGA

Reference
N/A

I. Information of Relaxase

ID	563
Name	Mob_pUR3937
GenBank accession number	YP_006962162
Family	MOB_P
Length	336 aa
UniProt ID	I0B6K6
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.80E-79, Aligned region: 8..242
Note	putative relaxase
Protein sequence [Download]	MATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKAARELYGKTDGNQGHVIIQ SFQPGEVTPEQCNQLGLALAEKLVPNHQVAVYTHADTNHVHNHIVLNSIDLNTGKKFNNN KQALRDVRAFNDEVCRAQGLSVPEKDTARLRYTQTEKAIADPNTTSTAQYSWKDDIREAI DQSTATNMEEFQKHLNQHGITIERVTPKSITYRHLAENKKVRGRKLGEDYNKGGIQHGFE RQIQRRQQQKHKSDTNFQPKRSTSNRHESTERDTGVTQSDWDQFAQDTNELERRRQAAES ARLVDEKARRDREKRVREKPTSRPIINIDRDHGLEL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	953
Plasmid name	pUR3937
GenBank accession number	NC_019304.1
Genome size	7082 bp
Coordinate of oriT [Strand]	4..207 [-]
Drug resistance	resistance to streptogramin A
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Staphylococcus epidermidis C3937 [1282]

Reference

[1] Lozano C et al (2012) Identification of novel vga(A)-carrying plasmids and a Tn5406-like transposon in meticillin-resistant Staphylococcus aureus and Staphylococcus epidermidis of human and animal origin. Int J Antimicrob Agents. 40(4):306-12. [PMID:22901706]