oriTDB

The information of the oriT region

oriTDB accession number	100490
Name	oriT_pWZ7140
Sequence Completeness	incomplete
oriT length	47 nt
oriT sequence	GTCATAGAATAATTCTTTGTTTTCTTCAATCGATTCAATAGAAATAT
IR (inverted repeat)	IR1: [3-10] [12-19] nt (CATAGAAT..ATTCTTTG) IR2: [25-31] [32-38] nt (TTCAATC..GATTCAA)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	predicted by the oriTfinder
	L:3-10;R:12-19;L:25-31;R:32-38

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GTCATAGAATAATTCTTTGTTTTCTTCAATCGATTCAATAGAAATAT

Reference
N/A

I. Information of Relaxase

ID	561
Name	Nickase_pWZ7140
GenBank accession number	YP_006961949
Family	MOB_Q
Length	654 aa
UniProt ID	E0X5B7
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 4.10E-67, Aligned region: 11..235
Note	putative relaxase
Protein sequence [Download]	MTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDR QTLWNKMELAEKSPNAQLCREVNVALPIELNNSDQRMLIEDFVKDNFVSEGMIADVAIHR DDENNPHAHIMLTMREVDSEGNILNKRKRIPKLDENGNQIFNEKGQRVTVSIKTNDWDRK SLVSEIRKDWADKVNQYLKDRNIDQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISE LKRKNLEIQSYNDVLSELDKLENQEKVLKQDQNFTLKFEKTFSPLEKKELKNLSKELKIF VNDENIDKRISELKRWENSLIFNNKMEIQKQRLMLSKISSERDMLTKANEILDKQAERFF KKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVVEKEESKKIFKEKPF QTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVS IDSVIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQ GVFFVQDSMKHIDELSPLAKHNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDV PEKNEVTVKMFQFAKSINRLLSGNQLQKKRNLDKLIKQTKAKENQSLQRNIPLR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	951
Plasmid name	pWZ7140
GenBank accession number	NC_019284.1
Genome size	47277 bp
Coordinate of oriT [Strand]	34415..34461 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Enterococcus faecalis [1351]

Reference

[1] Zhu W et al (2010) Dissemination of an Enterococcus Inc18-Like vanA plasmid associated with vancomycin-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 54(10):4314-20. [PMID:20660665]