oriTDB
The information of the oriT region
oriTDB accession number 100482
Name oriT_pSP02  insolico
Sequence Completeness core
oriT length 35 nt
oriT sequence TGAATGTTACCACCGGTAACATGTAAGTGCGCCCT
IR (inverted repeat)[4-11] [15-22] nt  (ATGTTACC..GGTAACAT)
Location of nic site [31-32] nt
Conserved sequence flanking the
  nic site 		TAAGTGCG|CCCT
Note predicted by the oriTfinder
L:4-11;R:15-22;D:31-32
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 553
Name MobA_pSP02 insolico
GenBank accession number YP_006960409
Family MOBQ
Length 370 aa
UniProt ID A0ES90
PDB ID _
Pfam MobA_MobL [PF03389.14], Evalue: 2.50E-56, Aligned region: 1..224
Note putative relaxase
Protein sequence [Download] MRDERTGEAFNGFGRRERVEHVCTMLPEGAPGEYLDPERLFNAVEMAEKRSDARPAKKIM
VALPREFDARERFRALEDFISWNITANGYACTYAIHTDKDGRNPHAHILVANRRIDPKTG
RWAAKSRSEFALDANGRRIPVIDPDTGRQKIGARNRKVWKRVNVSNNPLDSKEFLERLRR
EWADSCNALLPGYAVIDHRSFKARGIERIPTIHEGYASREMEKRGGRGDLCEENRRIQAL
NRLLDALRAMIGRLSDQAQGILTAVKRRQRPSGAPQAPSSPEPAERPQERPQARETPPQA
PQEPPKPIRTKKALTDRFKTRLDERLAENERKQAEKQAEPDMEETWDLSDPADPLNIGMG
WGTGGHGLGL
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 943
Plasmid name pSP02
GenBank accession number NC_019200.1
Genome size 4896 bp
Coordinate of oriT  [Strand] 3065..3099 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Bifidobacterium longum M62 [216816]
Reference
[1] Alvarez-Martín P et al (2013) Sequence analysis of plasmid pSP02 from Bifidobacterium longum M62 and construction of pSP02-derived cloning vectors. Plasmid. 69(2):119-26. [PMID:23228478]

Microbial Bioinformatics Group in MML, SJTU