oriTDB

The information of the oriT region

oriTDB accession number	100482
Name	oriT_pSP02
Sequence Completeness	core
oriT length	35 nt
oriT sequence	TGAATGTTACCACCGGTAACATGTAAGTGCGCCCT
IR (inverted repeat)	[4-11] [15-22] nt (ATGTTACC..GGTAACAT)
Location of nic site	[31-32] nt
Conserved sequence flanking the nic site	TAAGTGCG\|CCCT
Note	predicted by the oriTfinder
	L:4-11;R:15-22;D:31-32

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TGAATGTTACCACCGGTAACATGTAAGTGCGCCCT

Reference
N/A

I. Information of Relaxase

ID	553
Name	MobA_pSP02
GenBank accession number	YP_006960409
Family	MOB_Q
Length	370 aa
UniProt ID	A0ES90
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 2.50E-56, Aligned region: 1..224
Note	putative relaxase
Protein sequence [Download]	MRDERTGEAFNGFGRRERVEHVCTMLPEGAPGEYLDPERLFNAVEMAEKRSDARPAKKIM VALPREFDARERFRALEDFISWNITANGYACTYAIHTDKDGRNPHAHILVANRRIDPKTG RWAAKSRSEFALDANGRRIPVIDPDTGRQKIGARNRKVWKRVNVSNNPLDSKEFLERLRR EWADSCNALLPGYAVIDHRSFKARGIERIPTIHEGYASREMEKRGGRGDLCEENRRIQAL NRLLDALRAMIGRLSDQAQGILTAVKRRQRPSGAPQAPSSPEPAERPQERPQARETPPQA PQEPPKPIRTKKALTDRFKTRLDERLAENERKQAEKQAEPDMEETWDLSDPADPLNIGMG WGTGGHGLGL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	943
Plasmid name	pSP02
GenBank accession number	NC_019200.1
Genome size	4896 bp
Coordinate of oriT [Strand]	3065..3099 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Bifidobacterium longum M62 [216816]

Reference

[1] Alvarez-Martín P et al (2013) Sequence analysis of plasmid pSP02 from Bifidobacterium longum M62 and construction of pSP02-derived cloning vectors. Plasmid. 69(2):119-26. [PMID:23228478]