oriTDB

The information of the oriT region

oriTDB accession number	100475
Name	oriT_pSD4.6
Sequence Completeness	intact
oriT length	174 nt
oriT sequence	GGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTAC TATGTTGGCACGGATGCGAGTGTCAGTGAAGTGCTTCATGTGGCAGGAGAAAAATGCAGC AGCGGAGCGTCAGCAGAATATGTGATACAGGATATATTCCGCTTCCTCGCTCAC
IR (inverted repeat)	[2-11] [15-24] nt (GTTTCGGGGC..GCCCTGAACC)
Location of nic site	[55-56] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:2-11;R:15-24;D:55-56

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTAC

61

TATGTTGGCACGGATGCGAGTGTCAGTGAAGTGCTTCATGTGGCAGGAGAAAAATGCAGC

121

AGCGGAGCGTCAGCAGAATATGTGATACAGGATATATTCCGCTTCCTCGCTCAC

Reference
N/A

I. Information of Relaxase

ID	546
Name	MobA_pSD4.6
GenBank accession number	YP_006957906
Family	MOB_P
Length	499 aa
UniProt ID	K4KBN0
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 2.80E-16, Aligned region: 49..247
Note	putative relaxase
Protein sequence [Download]	MIVKFHPRGRGGGAGPVDYLLGKDRQREGATVLQGKPEEVRELIDASPYVKKYTSGVLSF AEADLPSGQREKLMASFERVLMPGLDKDQYSILWVEHRDKGRLELNFLIPNTELLTGRRL QPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSALPKAKQEAAEAITRGLLA LASSGELKTRQDVTEALESAGFEVVRTTKSSISIADPDGGRNIRLKGAIYEQSFNAGEGL RAEIESAAAEYRRDAESRIQRAREICKSGTERKREENQRRHPRPRPETVLSHEPAHERHG AHGQPDVADHRPGLRAADRLERGHSVVAGAADTRQLHDHPGAEEHAGHAVREEQRRAALD LRRAETPLREGEPGSGTVRRGFELDDTGGEIAHDGAGKTVAERIRAATAGLLEKAGRVGE RLRGMADDVWSYATGERSAERARHGLEQAGAEFERAAAPVVERLNAVELHREQERVVQHQ KALELERSQRQRTYNSPSL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	936
Plasmid name	pSD4.6
GenBank accession number	NC_019135.1
Genome size	4664 bp
Coordinate of oriT [Strand]	4254..4427 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Salmonella enterica subsp. enterica serovar Derby [28144]

Reference

[1] Bleicher A et al (2013) The plasmidome of a Salmonella enterica serovar Derby isolated from pork meat. Plasmid. 69(3):202-10. [PMID:23333216]