oriTDB

The information of the oriT region

oriTDB accession number	100473
Name	oriT_pSGSC3045-121
Sequence Completeness	intact
oriT length	360 nt
oriT sequence	GATTATCAAGAGTCACATGTGAATCGGCCATTTATTTTATTAGAGTCAACCTGGATTCAC CAACTTCATACAGGCGAATCTGTTATACCGAAATTAAGGATTCGAATCCAGATTCGAATC CTTAAATTTGTCTTAATTTAGTGGTCTGATTCGTAGATGAATCTGGATTCAACTCCTTTA CGATTCACTAAAGATTTGCATGTGAATCTAGTATCTATATCGGACACAGAATAACTATAA AAATATTAACCTAACATACTGAAATAAAAAGATAATCATGCAATATTAAATCTTGCGACG GGTGTGGGATTTTGATGTTAAGGAGCAGGCATACAGAAATATGTCAGCCAGGAAGAATTC
IR (inverted repeat)	[280-287] [289-296] nt (GCAATATT..AATCTTGC)
Location of nic site	[305-306] nt
Conserved sequence flanking the nic site	GACGGGTGT\|GG
Note	predicted by the oriTfinder
	L:280-287;R:289-296;D:305-306

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GATTATCAAGAGTCACATGTGAATCGGCCATTTATTTTATTAGAGTCAACCTGGATTCAC

61

CAACTTCATACAGGCGAATCTGTTATACCGAAATTAAGGATTCGAATCCAGATTCGAATC

121

CTTAAATTTGTCTTAATTTAGTGGTCTGATTCGTAGATGAATCTGGATTCAACTCCTTTA

181

CGATTCACTAAAGATTTGCATGTGAATCTAGTATCTATATCGGACACAGAATAACTATAA

241

AAATATTAACCTAACATACTGAAATAAAAAGATAATCATGCAATATTAAATCTTGCGACG

301

GGTGTGGGATTTTGATGTTAAGGAGCAGGCATACAGAAATATGTCAGCCAGGAAGAATTC

Reference
N/A

I. Information of Relaxase

ID	544
Name	Putative relaxase of pSGSC3045-121
GenBank accession number	YP_006957547
Family	MOB_F
Length	1770 aa
UniProt ID	I3W4H4
PDB ID	_
Pfam	TrwC [PF08751.10], Evalue: 1.10E-71, Aligned region: 7..280 TraI [PF07057.10], Evalue: 3.20E-46, Aligned region: 1444..1565 AAA_30 [PF13604.5], Evalue: 3.10E-34, Aligned region: 970..1162 AAA_19 [PF13245.5], Evalue: 8.40E-10, Aligned region: 975..1111
Note	putative relaxase
Protein sequence [Download]	MLSISSIKGDAGYYSHEDNYYASGSLDSRWMGEGAEKLGLKGEVASADMDAVRQGRLPDG SDLSRMVDGVNKHRSGYDLTFSAPKSVSVMALVGEDRRFIEAHNRAVAVVMKEVEQLVSA RITQEGKTETVLTGSMVAALYNHDTSRDLDPQVHTHALVFNATFADEKWRSLASDTRMKT GFSENLYATKIALGNLYRSALREDIESMGFETVAAGKHGLWELKDVPVDIFSSRSQAIRE AAGPDASAKSRDVAALDTRQAKAWADPDLLKADWRRRLTDEKFDIGHYISQAQARVEITG PVVAGQGGMRAPGQPGIASSGEAADELVQKAVSDTISALSDKKVQFTWSEMLAGTVNRLP STPGLFELARAGIEAAIEGLRLIPLDREKGIFTSDIHLLNELSVHQLARTAVAEQTVLVF PERAQERDIPAGDAVSVLSQDKSPVAILSGRGGAQTLRERTEDVAMMARSQGREVMVIAA DGRSGQFLSESPHLAGHVMLRSQMNADTVLPVQGTVIVDRAERLSLKETVLLQEKALSAG AQLIFMDTENRQGTGNALSVLKEADIPQYRFYGTQLPEVRLVSEADKRSRYSQLAQDYVR LSAEGRDVVAQVTGAREQQQLTEAIRDTRREAGELGREQVTLRVLEPVWLDSKTRLQRDN YRPGMVMEQWDAEKKTMTRHTIDRVAEATNSLVLQGEDGTRLTLKVTQLDGSWSLYRSRT LEVSEGDRVRALGRELKGAIKAKEQFTVAGLENGAVRLRSGDRELRLPTERAVKLTHDYV EGTGAGTNASRTVLAAVGPRGLNKQALNALAQSGSDIRIYTPLEGRQAARKVESVSAVQL ASDQVRQSTGEANLDTAIQASRERLMSDAEQAVNLAIPRAQQGQVYLSELTLLAEAVKSG QPLADVRAEIARQVDSGALIKLASVAGAGNRVLVPRIAYEMEKTIIRHIAEGKDAVQPLM ALTPASVLSGLTTGQREATRTVLENTDRFMAIQGYAGVGKTTQFRAVMGALNTLSESVRP QVIGLGPTHRAVHEMREAGVEARTLASFLSETRLAIQAGEIPDFRNVLFLTDESSMVGNR DMAELYQLVAAGGGRMVSSGDTAQLQAISTGLPFRLVQQRSAIDTVVMQEIVRQTPALRP AIESIIAGQVEISLRQVDDVTPQQVPRQPGAWVPDNSVMEIRAPKKDQEQDQEQGYLVAA GEQTLTPEQLALVRTDIIEAIRDDWMGRTPEAQQQTLVVAELNADRHAINDAIHTARHEK GDIGAEERTFTVLEPLRVPDNALRAAETFAEYAGAVAMMNERYWTVAEVDTQDAVVTLRN ADGESVLISPQQNTAQDISLFTPRDLTISQGDRVRFTRSDTDRGYVANSLWEVAGFTDEG AIRFRQGDQEKIVDPQAMTEDRHIDLAYALTVYGVQGASECFAIALTGTEGGRKRMVSLE STYVTLSRAKEHVQVYTDNLAGWSADARHSNAGQTAHDLLHQKSDHESDTGNRLLATASR LDKTALGRRVLAENGLEGETMARFIAAGKKYPSPYVALPAWTRHGKAAGALLTEIRIEDD GMRVVLSDESRLRGGEDAQFAGLQASRNGQTLIADDAQTALRLAQENPESGVVIRLHGEE RLLNAARLTGGRITEPDEVARTVRSVAEAESVAKVEDPITLPPDEQQKLAEAQEKAAREL AEQARQELLPAVPGEESTRPDPLLSADEGRRLRDGTARGERELDEAIQEAVADGRGIRQQ VREQMLRTEREWVVNVPEKDIELEKTLGGD

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	934
Plasmid name	pSGSC3045-121
GenBank accession number	NC_019125.1
Incompatibility group	IncFII(Yp)
Genome size	108459 bp
Coordinate of oriT [Strand]	79628..79987 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	virulence associated protein
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Salmonella enterica subsp. salamae SGSC3045 [59202]

Reference
N/A