oriTDB
The information of the oriT region
oriTDB accession number 100457
Name oriT_Plm  insolico
Sequence Completeness intact
oriT length 110 nt
oriT sequence TCACTTCAGGCTCCTTACGGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATA GCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTCAGG
IR (inverted repeat)IR1: [24-31] [35-42] nt  (GTCGGGGC..GCCCTGAC)
IR2: [49-65] [72-88] nt  (GCAATTGTAATAGCGTC..GACGGTATTACAATTGC)
Location of nic site [96-97] nt
Conserved sequence flanking the
  nic site 		CATCCTG|T
Note predicted by the oriTfinder
L:24-31;R:35-42;L:49-65;R:72-88;D:96-97
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 528
Name Nuclease_Plm insolico
GenBank accession number YP_006954408
Family MOBP
Length 899 aa
UniProt ID I7B2W8
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 4.20E-63, Aligned region: 47..315
Note putative relaxase
Protein sequence [Download] MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSPSSQAEQPYRSRFSRLVDYATRI
RNELFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHY
ILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYL
NRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRIVRKTAVERDRQNAWTRGKKQTF
REYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGKFLVMDGWDRNREGVQLDSFG
PSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPGKIAETESLQQYACRHL
GERLPEMAREGRLENCQAIHRTLAEVGLWMRVQHGHLVICDGYDHTQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFSGRRMESCPATDKEWHRMRTGTGPQGAIKREL
FSDKESLWGYSISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHE
QTPVKASSIHPDLTLRRAEPQAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDP
MLRRQRREARAEARADLRARYLAWREQWRKPDLRYGERCREIHQACRLRKSHIRAQYNDP
ALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVS
QLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGE
LVCTDYGDRVVFRNHHDRNALADKLYLIAPVLFERDPRMGFEPEGNDGQFNQVFAEMVAW
HNVTGFSEHGNYVITRPDVDQHREVSERGYRNYMDSNTYRDGTQPVQDSENRWEPPTPV
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 918
Plasmid name Plm
GenBank accession number NC_019097.1
Incompatibility group IncI1
Genome size 98809 bp
Coordinate of oriT  [Strand] 37457..37566 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli EC25 [562]
Reference
[1] Eberhart LJ et al (2012) Characterization of a novel microcin that kills enterohemorrhagic Escherichia coli O157:H7 and O26. Appl Environ Microbiol. 78(18):6592-9. [PMID:22773653]

Microbial Bioinformatics Group in MML, SJTU