oriTDB

The information of the oriT region

oriTDB accession number	100455
Name	oriT_p417H-90
Sequence Completeness	intact
oriT length	450 nt
oriT sequence	ATATGTATACCTCTTTATTTTCTTATCGCATCAAATTTATTTCATATATAAAAAAATGAC AATTCATCGATGAATCGAATCGTGACGCAAGTTACGAAAATTACGATTCATGCAGCAAAT CACATCACGAATTGAATCTAGAGTCAATTCGCTTTAACTCGTTGTTTTATTATTACTGAT TCATCTATGAGTTGTGTTAAATTCATTGGTGAATCATATGCGATTCACCAATGAATCCTT TTTATAATGTAAAATAAATTAAAATACATTATTTAAAACATAAGTTAATGATTCAAATAG CAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCACCA ACTAGTTGAGCCTTATTGTGGAGTAGGTTAAATTATTTCCGGATAATGTCACCAGAGGTG AAAAAATGAAAAAGTGGATGTTAGCAATCT
IR (inverted repeat)	IR1: [201-215] [222-236] nt (ATTCATTGGTGAATC..GATTCACCAATGAAT) IR2: [308-315] [318-325] nt (GCAAAAAC..GTTTTTGC)
Location of nic site	[334-335] nt
Conserved sequence flanking the nic site	GTAGTGTGT\|GG
Note	predicted by the oriTfinder
	L:201-215;R:222-236;L:308-315;R:318-325;D:334-335

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

ATATGTATACCTCTTTATTTTCTTATCGCATCAAATTTATTTCATATATAAAAAAATGAC

61

AATTCATCGATGAATCGAATCGTGACGCAAGTTACGAAAATTACGATTCATGCAGCAAAT

121

CACATCACGAATTGAATCTAGAGTCAATTCGCTTTAACTCGTTGTTTTATTATTACTGAT

181

TCATCTATGAGTTGTGTTAAATTCATTGGTGAATCATATGCGATTCACCAATGAATCCTT

241

TTTATAATGTAAAATAAATTAAAATACATTATTTAAAACATAAGTTAATGATTCAAATAG

301

CAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCACCA

361

ACTAGTTGAGCCTTATTGTGGAGTAGGTTAAATTATTTCCGGATAATGTCACCAGAGGTG

421

AAAAAATGAAAAAGTGGATGTTAGCAATCT

Reference
N/A

I. Information of Relaxase

ID	526
Name	TraI_p417H-90
GenBank accession number	YP_006954170
Family	Other
Length	1640 aa
UniProt ID	I3W000
PDB ID	_
Pfam	TraI [PF07057.10], Evalue: 2.00E-56, Aligned region: 1318..1440 TrwC [PF08751.10], Evalue: 4.40E-39, Aligned region: 1..168 AAA_30 [PF13604.5], Evalue: 1.50E-34, Aligned region: 852..1044 AAA_19 [PF13245.5], Evalue: 6.80E-09, Aligned region: 857..992
Note	putative relaxase
Protein sequence [Download]	MEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHAVVANVTQHNGEWKTLS SDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVVGKHGMWEMPGVPVEAFSG RSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEIKMAEWMQTLKETGFDIRAYRDAAD QRADLRTLTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPEAGVIER ARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRT AGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNL KQDERLSGELITGRRQLQEGMTFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITD SGQRTGTGSALMAMKDAGVNIYRWQGGEQRPTTIISEPDRNVRYARLAGDFAASVKAGEE SVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDSRSRYLRDMYRPGMVM EQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDSSWSLFRPEKMPVADGE RLRVTGKIPGLRVSGGDRLQVTSVSEDAMTVVVPGRAEPASLPVSDSPFTALKLENGWVE TPGHSVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVV SEQIKTRAGETSLETAISHQKSALHTPAQQAIHLALPVVESKNLAFSQVDLLTEAKSFAA EGTSFTDLGGEINAQIKRGDLLHVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTP LMERVPGELMEKLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPERE RPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVG NTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPEL REAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMQ KGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGK EQVMVPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNT RLISPREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSD GQKTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKSDREVMNAERLFSTARELRDVAA GRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAGIWLNPLTTDDGNGLRG FSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNP GAITGGRVWGDIPDNSVQPGAGNGEPLTAEILAQRQAEEAIRRETERRADEIVRKMAENK PDLPDGKTEQAVREIAGQERDQAVTSEREAALPESVLREPQREREAVREIARENLLQERL QQMERDMVRDLQKEKTLGGD

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	916
Plasmid name	p417H-90
GenBank accession number	NC_019094.1
Incompatibility group	IncFIC(FII)
Genome size	79478 bp
Coordinate of oriT [Strand]	77317..77766 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli 417H [562]

Reference
N/A