oriTDB

oriT
Relaxosome
T4CP
Plasmid&Host

The information of the oriT region

oriTDB accession number	100443
Name	oriT_pNDM-HK
Sequence Completeness	core
oriT length	106 nt
oriT sequence	AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGT CTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
IR (inverted repeat)	[18-35] [41-58] nt (GTTTTTGGAGTACCGCCG..CGGCGGCCGTCCAAAAAC)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	predicted by the oriTfinder
	L:18-35;R:41-58

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	514
Name	Nickase_pNDM-HK
GenBank accession number	YP_006952501
Family	MOB_P
Length	659 aa
UniProt ID	F1CNQ2
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 3.40E-32, Aligned region: 13..334
Note	putative relaxase
Protein sequence [Download]	MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETVLAVKSDMFEGLNNYLIR KQKVISTPVDVEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQNTRCKDPVMHYVLSW PDYEKPNDDQVFDSVKFTLASMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSS FTKDTLHQACRLLELKNGWSHSNGAYVVNDRQQIVRNPHSKKERGNWRSLDRINKMENKE GVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHKTFADIGLRVEKAQGKKGYVI THEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTAYTPGAYRRDA NKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAAL SQMRGFAYSSRKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGT VIFERDGKPVAADRGHIVLTESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCN RIVDAAVNHNLPVAQGITFTDAAQQAYAQNEKHRLIREQNNSKNEMQFRSESDDKFNPK

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	904
Plasmid name	pNDM-HK
GenBank accession number	NC_019063.1
Incompatibility group	IncL/M
Genome size	88803 bp
Coordinate of oriT [Strand]	65764..65869 [+]
Drug resistance	quaternary ammonium compound-resistance; sulfonamide resistance
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli HK-01 [562]

Reference

[1] Ho PL et al (2011) Complete sequencing of pNDM-HK encoding NDM-1 carbapenemase from a multidrug-resistant Escherichia coli strain isolated in Hong Kong. PLoS One. 6(3):e17989. [PMID:21445317]