oriTDB

oriT
Relaxosome
T4CP
Plasmid&Host

The information of the oriT region

oriTDB accession number	100442
Name	oriT_pPWD4_103
Sequence Completeness	intact
oriT length	110 nt
oriT sequence	TCACTTCAGGCTCCTTACGGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATA GCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTCAGG
IR (inverted repeat)	IR1: [24-31] [35-42] nt (GTCGGGGC..GCCCTGAC) IR2: [49-65] [72-88] nt (GTAATTGTAATAGCGTC..GACGGTATTACAATTAC)
Location of nic site	[96-97] nt
Conserved sequence flanking the nic site	CATCCTG\|T
Note	predicted by the oriTfinder
	L:24-31;R:35-42;L:49-65;R:72-88;D:96-97

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	513
Name	NikB_pPWD4_103
GenBank accession number	YP_006952344
Family	MOB_P
Length	871 aa
UniProt ID	E9LMH8
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 5.80E-62, Aligned region: 20..287
Note	putative relaxase
Protein sequence [Download]	MTDEELDLSSSSQAEQPHRNRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFH NCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVRHTLKSLGL GEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQETLSRACRELELKHGFAPDNG CWVHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLA EDGLYLSQMDGKFLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVDA PGRYNPDFIAADVRPEKIAETESLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGL WMRVQHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWQPVPTDIFRQVTPTERF RGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEF TWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFVSA PADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQW RKPDLRYGERCREIHQACRLRKSHIRAQYDDPVLRKLHYHIAEVQRMQALIRLKEDIRDE RQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCE PGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLI APVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITRPDVDHHREGSER YYRDYIAANSNDDASLPPPEQDKRWEPPSPG

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	903
Plasmid name	pPWD4_103
GenBank accession number	NC_019061.1
Incompatibility group	IncI1
Genome size	103297 bp
Coordinate of oriT [Strand]	43373..43482 [-]
Drug resistance	hygromycin resistance; apramycin resistance
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli PWD4 [562]

Reference

[1] Johnson TJ et al (2011) Comparative genomics and phylogeny of the IncI1 plasmids: a common plasmid type among porcine enterotoxigenic Escherichia coli. Plasmid. 66(3):144-51. [PMID:21843549]