oriTDB
The information of the oriT region
oriTDB accession number 100439
Name oriT_pND12_96  insolico
Sequence Completeness intact
oriT length 110 nt
oriT sequence TCACTTCAGGCTCCTTACGGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATA GCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTCAGG
IR (inverted repeat)IR1: [24-31] [35-42] nt  (GTCGGGGC..GCCCTGAC)
IR2: [49-65] [72-88] nt  (GTAATTGTAATAGCGTC..GACGGTATTACAATTAC)
Location of nic site [96-97] nt
Conserved sequence flanking the
  nic site 		CATCCTG|T
Note predicted by the oriTfinder
L:24-31;R:35-42;L:49-65;R:72-88;D:96-97
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 510
Name NikB_pND12_96 insolico
GenBank accession number YP_006903442
Family MOBP
Length 899 aa
UniProt ID E9LLX8
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 1.40E-62, Aligned region: 47..315
Note putative relaxase
Protein sequence [Download] MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRL
RNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHY
ILSWQAHESPRPEQIYDSVRHTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYL
NCLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRIVRKTAVERDRQNAWTRGKKQTF
REYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGKFLVMDGWDRNREGVQLDSFG
PSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETESLQQYACRHL
GERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFLQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKREL
FSDKESLWGYSISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHE
QTPVKASSIHPDLTLGRAEPQAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDP
MLRRQRREARAEARADLRARYLAWREQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDP
ALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVS
QLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGE
FVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 900
Plasmid name pND12_96
GenBank accession number NC_019044.1
Incompatibility group IncI1
Genome size 92290 bp
Coordinate of oriT  [Strand] 34167..34276 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli ND12 [562]
Reference
[1] Johnson TJ et al (2011) Comparative genomics and phylogeny of the IncI1 plasmids: a common plasmid type among porcine enterotoxigenic Escherichia coli. Plasmid. 66(3):144-51. [PMID:21843549]

Microbial Bioinformatics Group in MML, SJTU