oriTDB

The information of the oriT region

oriTDB accession number	100433
Name	oriT_pHUSEC41-3
Sequence Completeness	intact
oriT length	88 nt
oriT sequence	TCAGGGGGTTTCGGGGCGAAGCCCTGAACCAGTCACGTAGCGATAGCGGAGTGTATACTG GCTTAACTATGCGGCATCAGTGCAGATT
IR (inverted repeat)	[8-17] [21-30] nt (GTTTCGGGGC..GCCCTGAACC)
Location of nic site	[61-62] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:8-17;R:21-30;D:61-62

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TCAGGGGGTTTCGGGGCGAAGCCCTGAACCAGTCACGTAGCGATAGCGGAGTGTATACTG

61

GCTTAACTATGCGGCATCAGTGCAGATT

Reference
N/A

I. Information of Relaxase

ID	504
Name	Mob_pHUSEC41-3
GenBank accession number	YP_006940173
Family	MOB_P
Length	517 aa
UniProt ID	G4VUT8
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.90E-19, Aligned region: 49..246
Note	putative relaxase
Protein sequence [Download]	MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGDPEEVRELIDATPFAKKYTSGVLSF AEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRL QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRILTLPDNLPETKQALAESVTRGIDA LYHVGEIKGRQDVIQALTEAGLEVVRVTRSSISIADPNGGKNIRLKGAFYEQSFTDGRGV REKAERESRIYRDNAERRVQQARKICKQGCDIKRDENQRRYSPVHSFDRGITEKTPGRGE RGDDAAQEGRVKAGREYGHDVTGDGHFPVYREWRDALVSWRDDTGEPGRNQDTGRNIAET EREDMGRGVCAGREKEISGYPAGEIGNGDSLSGEGLGTTDGVTQSDGAGKTFAERLRATA TGLYAAAERMGERLRGIAENVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREQ EIRDERLIQEREHELSLERERQPEIQERTLDGPSLGW

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	894
Plasmid name	pHUSEC41-3
GenBank accession number	NC_018997.1
Incompatibility group	ColRNAI
Genome size	7930 bp
Coordinate of oriT [Strand]	827..914 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli HUSEC41 [562]

Reference

[1] Künne C et al (2012) Complete sequences of plasmids from the hemolytic-uremic syndrome-associated Escherichia coli strain HUSEC41. J Bacteriol. 194(2):532-3. [PMID:22207742]