oriTDB

The information of the oriT region

oriTDB accession number	100431
Name	oriT_pEAR4.3
Sequence Completeness	intact
oriT length	185 nt
oriT sequence	GGTTTTAGCGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTAT ACTGGCTTAATATGTTACGGCGAAGTGGTGATTAATGGAAGTGCTTCATGTGGCAGGGGA AATATGTGACTCAGGAGTGACCGCAATACGATGTACGGACAGATGAAATGACGCCTCCTC GCTCA
IR (inverted repeat)	[14-21] [25-32] nt (TTCGGGGC..GCCCTGAA)
Location of nic site	[65-66] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:14-21;R:25-32;D:65-66

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGTTTTAGCGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTAT

61

ACTGGCTTAATATGTTACGGCGAAGTGGTGATTAATGGAAGTGCTTCATGTGGCAGGGGA

121

AATATGTGACTCAGGAGTGACCGCAATACGATGTACGGACAGATGAAATGACGCCTCCTC

181

GCTCA

Reference
N/A

I. Information of Relaxase

ID	502
Name	MbeA_pEAR4.3
GenBank accession number	YP_006939437
Family	MOB_P
Length	509 aa
UniProt ID	E5BAY8
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 4.60E-15, Aligned region: 58..255
Note	putative relaxase
Protein sequence [Download]	MQCWRRGGMMIVKFHARGRGGGSGPVDYLLGKERNREGACVLQGRPEEVRELIDASPYAK KYTSGVLSFAEADLPFGQREKLMESFERVLMPGLDKDQYSILWVEHRDKGRLELNFVIPN TELLTGKRLQPYFDRADRPRIDAWQTIVNDLLELHDPNAPENRRALVTPGSLPESKQEAA RAITRGLLALASSGELKTRQDVTDALTASGFDVVRTTKSSISIADPDGGRNIRLKGAIYE QSFNAGEGLRAEIESAAAEYRRDAESRVQRARAVCKSGTERKREENQRRHQRPRAGYDLG HAVEQAERSAHGGPDVADHHDGVSAAYRQPERDTVVPGAAHRLEPGDHPGTGADAGHAVG EEQRGTAFDVRGQQTALREGEPGSGEVRRGFGLDDTGGEIEHDGAGKTVAERVRAATAGL LAKAGRVGERLRGMAEDVWSYATGERGAERSRIGLEQAGAEFERAASPLVDRLNVIEQRR EHERALQHQKTLELERSQRQRTYDGPSLG

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	892
Plasmid name	pEAR4.3
GenBank accession number	NC_018977.1
Genome size	4369 bp
Coordinate of oriT [Strand]	3850..4034 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Erwinia amylovora ATCC BAA-2158 [889211]

Reference

[1] Powney R et al (2011) Genome sequence of an Erwinia amylovora strain with pathogenicity restricted to Rubus plants. J Bacteriol. 193(3):785-6. [PMID:21131493]