oriTDB
The information of the oriT region
oriTDB accession number 100428
Name oriT_pAA-EA11  insolico
Sequence Completeness intact
oriT length 450 nt
oriT sequence ATATGTATACCTCTTTATTTTCTTATCGCATCAAATTTATTTCACATATAAAAAAATGAC AATTCATCGATGAATCGAATCGTGACGCAAGTTACGAAAATTACGATTCATGCAGCAAAT CACATCACGAATTGAATCTAGAGTCAATTCGCTTTAACTCGTTGTTTTATTATTACTGAT TCATCTATGAGTTGCGTTAAATTCATTGGTGAATCATATGCGATTCACCAATGAATCCTT TTTATAATGTAAAATAAATTAAAATACATTATTTAAAACATAAGTTAATGATTCAAATAG CAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCA ACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTG GAAAAATGAAAAAATGGATGTTAGCAATCT
IR (inverted repeat)IR1: [201-215] [222-236] nt  (ATTCATTGGTGAATC..GATTCACCAATGAAT)
IR2: [308-315] [318-325] nt  (GCAAAAAC..GTTTTTGC)
Location of nic site [334-335] nt
Conserved sequence flanking the
  nic site 		GTGGGGTGT|GG
Note predicted by the oriTfinder
L:201-215;R:222-236;L:308-315;R:318-325;D:334-335
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 499
Name TraI_pAA-EA11 insolico
GenBank accession number YP_006792604
Family Other
Length 1237 aa
UniProt ID A0A0E0Y6S8
PDB ID _
Pfam TraI [PF07057.10], Evalue: 1.80E-55, Aligned region: 986..1108
AAA_30 [PF13604.5], Evalue: 4.00E-34, Aligned region: 520..711
AAA_19 [PF13245.5], Evalue: 8.80E-09, Aligned region: 525..660
Note putative relaxase
Protein sequence [Download] MAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQDGMTFTPGSTVIVD
QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPA
TIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPE
VTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQG
ETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKTPGLRVSGGDRLQVASVSEDAMTVV
VPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARS
GRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISLQKAGLHTPAQQAI
HLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKRGDLLYVDVAKGYGT
GLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMILEASDRFT
VVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLH
DTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAP
GQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRLEG
AWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTL
IVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVPVLNTANIRDGELRRLSTWEKNPDALA
LVDSVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDKIRVGTGDRMRFT
KSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAITAHGAQGA
SETFAIALEGTEGNRKQMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHD
VFEPKPDREVMNAQRLFSTARELRDVAAGRAVLRQAGLSGGDSPARFIAPGRKYPQPYVA
LPAFDRNGRSAGIWLNPLTTDDGNGLRGFSGEGRVKGSEDAQFVALQGSRNGESLLADNM
QDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIPDSSVQPGAGNGEPVTAEVL
AQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTE
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 889
Plasmid name pAA-EA11
GenBank accession number NC_018666.1
Incompatibility group IncFIB(AP001918)
Genome size 74217 bp
Coordinate of oriT  [Strand] 52043..52492 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli O104:H4 str. 2011C-3493 [1133852]
Reference
[1] Ahmed SA et al (2012) Genomic comparison of Escherichia coli O104:H4 isolates from 2009 and 2011 reveals plasmid, and prophage heterogeneity, including shiga toxin encoding phage stx2. PLoS One. 7(11):e48228. [PMID:23133618]

Microbial Bioinformatics Group in MML, SJTU