oriTDB

The information of the oriT region

oriTDB accession number	100424
Name	oriT_pWTk445
Sequence Completeness	core
oriT length	52 nt
oriT sequence	AGCCTTTAAAGCGAAAATAGGGTACTCCATGCTCGCTATATCATCCCTGACA
IR (inverted repeat)	[17-25] [31-39] nt (ATAGGGTAC..GCTCGCTAT)
Location of nic site	[49-50] nt
Conserved sequence flanking the nic site	ATCCCTG\|A
Note	predicted by the oriTfinder
	L:17-25;R:31-39;D:49-50

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AGCCTTTAAAGCGAAAATAGGGTACTCCATGCTCGCTATATCATCCCTGACA

Reference
N/A

I. Information of Relaxase

ID	495
Name	Putative relaxase of pWTk445
GenBank accession number	WP_014752712
Family	MOB_P
Length	339 aa
UniProt ID	I3UHY4
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.60E-08, Aligned region: 95..254
Note	putative relaxase
Protein sequence [Download]	MKQLDQQVDKWFMEFKTRSIRPKPGAAGRGAMRRPLKPGTGLKNIQSAALKKPEVMVKIP PRKGGSNGIQAVRGHMDYISRNGTLDLEDQDGLIYKGQKQIHEGITNAWKALGVPEKSTK REAINLVLSMPPGTNPEAVKAAAREFAKEEFDGHQYAFVQHLDEKHPHVHLCVMMKHELG RRMDPRKADLFEWRLRFAEKMREQGVDCAATRRVHRGKTQKPEHSALRHMRKRGQVPNVY RQQAIELAQAVKGSTRPIHPFLREEIRTRNIVSTEYGKIAKELYKIGAKAEARVLSGFAK EIKLEGHTTQAQQRFEQAQGQIHEKTREDFSAHDKNIER

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	885
Plasmid name	pWTk445
GenBank accession number	NC_017965.1
Genome size	57884 bp
Coordinate of oriT [Strand]	40198..40249 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	VOC family virulence protein
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Advenella kashmirensis WT001 [1036672]

Reference

[1] Ghosh W et al (2011) Whole-genome shotgun sequencing of the sulfur-oxidizing chemoautotroph Tetrathiobacter kashmirensis. J Bacteriol. 193(19):5553-4. [PMID:21914874]