oriTDB
The information of the oriT region
oriTDB accession number 100424
Name oriT_pWTk445  insolico
Sequence Completeness core
oriT length 52 nt
oriT sequence AGCCTTTAAAGCGAAAATAGGGTACTCCATGCTCGCTATATCATCCCTGACA
IR (inverted repeat)[17-25] [31-39] nt  (ATAGGGTAC..GCTCGCTAT)
Location of nic site [49-50] nt
Conserved sequence flanking the
  nic site 		ATCCCTG|A
Note predicted by the oriTfinder
L:17-25;R:31-39;D:49-50
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 495
Name Putative relaxase of pWTk445 insolico
GenBank accession number WP_014752712
Family MOBP
Length 339 aa
UniProt ID I3UHY4
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 1.60E-08, Aligned region: 95..254
Note putative relaxase
Protein sequence [Download] MKQLDQQVDKWFMEFKTRSIRPKPGAAGRGAMRRPLKPGTGLKNIQSAALKKPEVMVKIP
PRKGGSNGIQAVRGHMDYISRNGTLDLEDQDGLIYKGQKQIHEGITNAWKALGVPEKSTK
REAINLVLSMPPGTNPEAVKAAAREFAKEEFDGHQYAFVQHLDEKHPHVHLCVMMKHELG
RRMDPRKADLFEWRLRFAEKMREQGVDCAATRRVHRGKTQKPEHSALRHMRKRGQVPNVY
RQQAIELAQAVKGSTRPIHPFLREEIRTRNIVSTEYGKIAKELYKIGAKAEARVLSGFAK
EIKLEGHTTQAQQRFEQAQGQIHEKTREDFSAHDKNIER
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 885
Plasmid name pWTk445
GenBank accession number NC_017965.1
Genome size 57884 bp
Coordinate of oriT  [Strand] 40198..40249 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor insolico VOC family virulence protein
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Advenella kashmirensis WT001 [1036672]
Reference
[1] Ghosh W et al (2011) Whole-genome shotgun sequencing of the sulfur-oxidizing chemoautotroph Tetrathiobacter kashmirensis. J Bacteriol. 193(19):5553-4. [PMID:21914874]

Microbial Bioinformatics Group in MML, SJTU