oriTDB

The information of the oriT region

oriTDB accession number	100423
Name	oriT_plasmid1_DO
Sequence Completeness	intact
oriT length	215 nt
oriT sequence	AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGC ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACAT TAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTA AAAGTTGACATACGCCTTTTTGATTGGAGGGATTT
IR (inverted repeat)	[61-76] [80-95] nt (ACTTAACCCCCCGTAT..ACAGGGGGGTACAAAT) [123-134] [139-150] nt (GAAAATCCTTTG..CAAGGGATTTAC)
Location of nic site	[135-136] nt
Conserved sequence flanking the nic site	TGG\|T
Note	predicted by the oriTfinder
	L:61-76;R:80-95;L:123-134;R:139-150;D:135-136

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGC

61

ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACAT

121

TAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTA

181

AAAGTTGACATACGCCTTTTTGATTGGAGGGATTT

Reference
N/A

I. Information of Relaxase

ID	494
Name	Pre_plasmid1_DO
GenBank accession number	YP_006377333
Family	MOB_V
Length	420 aa
UniProt ID	Q3Y252
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 1.70E-73, Aligned region: 1..193
Note	putative relaxase
Protein sequence [Download]	MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKE IIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIA YATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERG SDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVKSDLEVPAKRHMKSVEVPT GEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDNDRLKQHVRNLMSTDMAREYK KLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSLIYESTKEFLK ERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	884
Plasmid name	plasmid1_DO
GenBank accession number	NC_017961.1
Genome size	36262 bp
Coordinate of oriT [Strand]	23823..24037 [+]
Drug resistance	tetracycline resistance
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Enterococcus faecium DO [333849]

Reference

[1] Qin X et al (2012) Complete genome sequence of Enterococcus faecium strain TX16 and comparative genomic analysis of Enterococcus faecium genomes. BMC Microbiol. 0.59375. [PMID:22769602]