oriTDB

The information of the oriT region

oriTDB accession number	100422
Name	oriT_p666
Sequence Completeness	intact
oriT length	291 nt
oriT sequence	CGCACCGCTAGCAGCGCCCCTAGCGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAG CGCCCCTAATATAAAATGATGTTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAGCG GTGTCGGCGCGTTGTTGTAGCCGCGCCGACACCGCTTTTTTGAATATCATAAAGAGAGTA AGAGAAACTAATTTTTCATAACACTATATTTATAAAGAAAAATCAGCAAAAACTTGTTTT TGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCT
IR (inverted repeat)	[226-233] [236-243] n (GCAAAAAC..GTTTTTGC)
Location of nic site	[252-253] nt
Conserved sequence flanking the nic site	GTAGTGTGT\|GG
Note	predicted by the oriTfinder
	L:226-233;R:236-243;D:252-253

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CGCACCGCTAGCAGCGCCCCTAGCGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAG

61

CGCCCCTAATATAAAATGATGTTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAGCG

121

GTGTCGGCGCGTTGTTGTAGCCGCGCCGACACCGCTTTTTTGAATATCATAAAGAGAGTA

181

AGAGAAACTAATTTTTCATAACACTATATTTATAAAGAAAAATCAGCAAAAACTTGTTTT

241

TGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCT

Reference
N/A

I. Information of Relaxase

ID	493
Name	TraI_p666
GenBank accession number	WP_000948336
Family	MOB_F
Length	1756 aa
UniProt ID	E3PP28
PDB ID	_
Pfam	TrwC [PF08751.10], Evalue: 6.70E-76, Aligned region: 10..284 TraI [PF07057.10], Evalue: 5.20E-55, Aligned region: 1434..1556 AAA_30 [PF13604.5], Evalue: 2.40E-34, Aligned region: 968..1159 AAA_19 [PF13245.5], Evalue: 9.70E-09, Aligned region: 973..1108
Note	putative relaxase
Protein sequence [Download]	MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDIFTRLLEGRL PDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEAL ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHVVVANVTQHNGEWKTLSSDKV GKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVVGKHGMWEMPGVPVEAFSGRSQA IREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRDAADQRAE IRTQAPGSASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPENGVIERARAG IDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDE RLSGELITGRRQLQEGMIFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQR TGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVVQ VSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWN PETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRV TGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGH SVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI KARAGETSLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTG FTELGGEINAQIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMER VPGELMEKLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRV VGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDM ARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAV YSLINRDVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMQNGEA FPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM VPVLNTANIRDGELRRLSTWENNPDALALVDNVYHRIAGISRDDGLITLQDAEGNTRLIS PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQT RVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMK QHVQVYTDNRQGWTDAIHNSVQKGTAHDVFEPKPDREVMNAEWLFSTARELRDVAAGRAV LRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGE GRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAIT GGRVWGDIPDNSVQPGAGNGEPVTAEVLAQQQAEEAIRRETERRADEIVRKMAENKPDLP DGKTEQAVREIAGQERDRTATSERETALPESVLRESQREQEAVREVARENLLQERLQQME RDMVRDLQKEKTLGGD

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	883
Plasmid name	p666
GenBank accession number	NC_017722.1
Incompatibility group	IncFII(29)
Genome size	66681 bp
Coordinate of oriT [Strand]	28663..28953 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli ETEC H10407 [316401]

Reference

[1] Crossman LC et al (2010) A commensal gone bad: complete genome sequence of the prototypical enterotoxigenic Escherichia coli strain H10407. J Bacteriol. 192(21):5822-31. [PMID:20802035]