oriTDB

The information of the oriT region

oriTDB accession number	100421
Name	oriT_p52
Sequence Completeness	intact
oriT length	85 nt
oriT sequence	GCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCT TAACCATGCGGCATCAGTGCAGATT
IR (inverted repeat)	_
Location of nic site	[58-59] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	D:58-59

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCT

61

TAACCATGCGGCATCAGTGCAGATT

Reference
N/A

I. Information of Relaxase

ID	492
Name	Nuclease_p52
GenBank accession number	WP_000635817
Family	MOB_P
Length	517 aa
UniProt ID	E3PP05
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 7.70E-18, Aligned region: 47..246
Note	putative relaxase
Protein sequence [Download]	MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF AEKVLPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQSGKRL QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRILTLSDNLPETKQALAESVTRGIDA LYHVGEIKGRQDVIQALTEAGLEVVRVTRSSISIADPNGGKNIRLKGAFYEQSFTDGRGV REKAERESRIYRDNAERRVQQARKICKQGCDLKRDENQRRYSPVHNVDRGIAGKTPGRGE RGDDAAQEGRVKAGREYGHEVAGDSLSPVYREWRDALVSWRADTGEPGRNQDTGRNIAET EREDMGRGVCAGREKEISGPSVREIGNGDSLSGEGLGTTDGVTQSDGAGKTFAERIRATA TGLYAAAERMGERLRGIAENVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREQ EIRDERLIQEREHALSLERERQPEIQERTLDGPSLGW

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	882
Plasmid name	p52
GenBank accession number	NC_017721.1
Genome size	5175 bp
Coordinate of oriT [Strand]	4842..4926 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli ETEC H10407 [316401]

Reference

[1] Crossman LC et al (2010) A commensal gone bad: complete genome sequence of the prototypical enterotoxigenic Escherichia coli strain H10407. J Bacteriol. 192(21):5822-31. [PMID:20802035]