oriTDB

The information of the oriT region

oriTDB accession number	100420
Name	oriT_pSLT_SL1344
Sequence Completeness	intact
oriT length	259 nt
oriT sequence	CTCACCACCAAAAGCACCACACCCCACGCAAAATTAAAATTTTTCTGATTTGTTTTTAGT AACAGTGAGTTATGTTCATAATAATGTAATTTAATTTATTTTAATGTATTAAATAGCGGT GCAGGCACGTCGCCGGCACCGCTATGACGACGCTATACAGGGGTAGTACTGACTATTTTT ATAAAAAACATTGCATTATATCAGGGGCGCTGCTAGCGTCGCTGTGCCTGTTTTTAAGTA ACACCCCTGGGGGCGCTGC
IR (inverted repeat)	[115-128] [134-147] nt (ATAGCGGTGCCGGC..GCCTGCACCGCTAT)
Location of nic site	[241-242] nt
Conserved sequence flanking the nic site	GTGGGGTGT\|GG
Note	predicted by the oriTfinder
	L:115-128;D:134-147;D:241-242

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CTCACCACCAAAAGCACCACACCCCACGCAAAATTAAAATTTTTCTGATTTGTTTTTAGT

61

AACAGTGAGTTATGTTCATAATAATGTAATTTAATTTATTTTAATGTATTAAATAGCGGT

121

GCAGGCACGTCGCCGGCACCGCTATGACGACGCTATACAGGGGTAGTACTGACTATTTTT

181

ATAAAAAACATTGCATTATATCAGGGGCGCTGCTAGCGTCGCTGTGCCTGTTTTTAAGTA

241

ACACCCCTGGGGGCGCTGC

Reference
N/A

I. Information of Relaxase

ID	491
Name	TraI_pSLT_SL1344
GenBank accession number	WP_000986921
Family	MOB_F
Length	1752 aa
UniProt ID	A0A0H3NX93
PDB ID	_
Pfam	TrwC [PF08751.10], Evalue: 1.30E-76, Aligned region: 10..284 TraI [PF07057.10], Evalue: 6.80E-59, Aligned region: 1430..1553 AAA_30 [PF13604.5], Evalue: 1.10E-34, Aligned region: 967..1163 AAA_19 [PF13245.5], Evalue: 5.10E-09, Aligned region: 972..1107
Note	putative relaxase
Protein sequence [Download]	MMSIAQVRSAGSAAGYYSDRDNYYVLGSMEERWAGKGAEQLGLQGTVDKEVFTRVLEGRL PDGADLSRQQDGGNKHRPGYDLTFSAPKSVSLMAMLAGDKRLTEAHNQAVDIAVRQVEAL ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHAVVVNVTQHDGEWKTLSSDKV GKTGFIENVYANQIAFGKIYRAVLKEKVEALGYETEVVGKHGMWEMPGVPVEAFSSRSQA IREAVGEDASLKSRDVAALDTRKSKQHVDPEVKMAEWMQTLKDTGFDISAYRESADRRAE IQAAQPVPSQEQPDIQQAVTQAIAGLSDRKVQFTYTDVLARTVGMLPPEAGVIEKARAGI DEAISREQLIPLDREKGLFTSGIHVLDELSVRALSSDIMKQNRVTVHPEKSVPRTGSYSD AVSVLAQDRPSLAIISGQGGAAGQRERVAELTMMAREQGREVQIIVADRRSQTNLKQDER LSGELITGRRQLQEGMLFSPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRT GTGSALMAMKEAGVNSYRWQGRQQTPATVISEPDRNVRYARLAGDFVAAVKAGEESVAQV SGMREQAILAGMIRSELKTQGILGQQDTMMTALSPVWLDSRNRYLRDMYREGMVMEQWNP EKRSHDRYVIDRVTAQSHSLTLRDAQGETQMVRISALDSSWSLFRPEKMPVADGERLMVT GKIPGLRVSVGDRLQVSAVNDGVMTVTVPGRAEPASLPVGDSPFTALKLENGWVETPGHS VSDSAKVFASVTQMAMDNATLNSLARSGRDVRLYSSLDETRTAEKLSRHPSFTVVSEQIK ARAGEVSLETAISRQKAGLHTPAQQAIHLALPVVESKNLAFSQVDLLTEAKSFAAEGTGF ADLGREIDAQIKRGDLLYVDVAKGYGTDLLISRASYDAEKSILHHILEGKEAVTPLMERV PGELMETLTSGQRAATRMILETKDRFTVVQGYAGVGKTTQFRAVMSAVNLLPESERPRVV GLGPTHRAVGEMRSAGVEAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMA RAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELRDAVY SLINRDVNKALSGLENVKPVQVPRLKGAWAPENSVTEFSRLQERELAKAAQEAEKKGEAF PDVPVTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIQDALAKPGEQQVTVPVL TTANIRDGELRRLSTWEAHPGALALVDNVYHRIAGISKEDGLITLEDKAGNTRLISPREA AAEGVTLYNPETIRVGAGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGKQTRVVR PGQDRAEQHIDLAYAITAHSAQGASETFAIALEGTEGGRKQMAGFESAYVALSRMKQHVQ VYTDDRQGWVKAINSAEQKGTAHDVLEPKSEREMMNAERLFSTARELRDVAAGRAVLRNA GLAQGDSRARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGAGLRGFTGEGRVK GSEEAQFVALQGSRNGESLLAGSMQDGVRIARENPDSGVVVRIVGDGRPWNPVAITGGRV WGDIPDSNVQPGAGNGEPVTAEILAQRQAEEAVRRETERRADEIVRKMAEDKSDLPEEKT AQAVREIAGQEQDRMTPPEREPPLPESVLREPQRERETIREVARENRGRERLQQMEQEMV RDLQKEKTPGGD

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	881
Plasmid name	pSLT_SL1344
GenBank accession number	NC_017720.1
Incompatibility group	IncFIB(S)
Genome size	93842 bp
Coordinate of oriT [Strand]	32927..33185 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	Salmonella plasmid virulence protein
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344 [216597]

Reference

[1] Kröger C et al (2012) The transcriptional landscape and small RNAs of Salmonella enterica serovar Typhimurium. Proc Natl Acad Sci U S A. 109(20):E1277-86. [PMID:22538806]