oriTDB

The information of the oriT region

oriTDB accession number	100419
Name	oriT_pRSF1010_SL1344
Sequence Completeness	intact
oriT length	160 nt
oriT sequence	GGCCAGTTTCTCGAAGAGAAACCGGTAAGTGCGCCCTCCCCTACAAAGTAGGGTCGGGAT TGCCGCCGCTGTGCCTCCATGATAGCCTACGAGACAGCACATTAACAATGGGGTGTCAAG ATGGTTAAGGGGAGCAACAAGGCGGCGGATCGGCTGGCCA
IR (inverted repeat)	IR1: [3-12] [16-25] nt (CCAGTTTCTC..GAGAAACCGG) IR2: [39-44] [48-53] nt (CCCTAC..GTAGGG)
Location of nic site	[33-34] nt
Conserved sequence flanking the nic site	TAAGTGCG\|CCCT
Note	predicted by the oriTfinder
	L:3-12;R:16-25;L:39-44;R:48-53;D:33-34

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGCCAGTTTCTCGAAGAGAAACCGGTAAGTGCGCCCTCCCCTACAAAGTAGGGTCGGGAT

61

TGCCGCCGCTGTGCCTCCATGATAGCCTACGAGACAGCACATTAACAATGGGGTGTCAAG

121

ATGGTTAAGGGGAGCAACAAGGCGGCGGATCGGCTGGCCA

Reference
N/A

I. Information of Relaxase

ID	490
Name	MobA_pRSF1010_SL1344
GenBank accession number	WP_001395566
Family	MOB_Q
Length	709 aa
UniProt ID	C7FHN5
PDB ID	_
Pfam	RepB_primase [PF16793.4], Evalue: 1.80E-47, Aligned region: 396..643 MobA_MobL [PF03389.14], Evalue: 2.00E-33, Aligned region: 13..218
Note	putative relaxase
Protein sequence [Download]	MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESGHMPEFVERPADYWDA ADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGAERLPYTLAIHAGGGENPH CHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEALKPKAWLEQTREAWADHANRA LERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGRGIRTDRADVALNIDTANAQIID LQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTGRRSPAGHEPDPAGQRGAGGG VAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGVALEAMANRDAGFHDAYGGAA DRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQM MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREP ALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKD KHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLS RHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAER KPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	880
Plasmid name	pRSF1010_SL1344
GenBank accession number	NC_017719.1
Incompatibility group	IncQ1
Genome size	8688 bp
Coordinate of oriT [Strand]	5546..5705 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344 [216597]

Reference

[1] Kröger C et al (2012) The transcriptional landscape and small RNAs of Salmonella enterica serovar Typhimurium. Proc Natl Acad Sci U S A. 109(20):E1277-86. [PMID:22538806]