 oriTDB accession number | 100414 |
| Name | oriT_pRK2  |
Sequence Completeness  | intact |
| oriT length | 175 nt |
| oriT sequence | GGTTTCGGGGCGAAGCCCTGAACCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAC TATGTTGGCATGGATGCGGAAATCAGTGAAGTGCTTCATGTGACAGGAGAAAAAAGGCGG CACAGGCGCGTCAGCAGAATATGCGATACAGGATATATTCCGCTTCCTCGCTCAC |
| IR (inverted repeat) | [2-11] [15-24] nt (GTTTCGGGGC..GCCCTGAACC) |
| Location of nic site | [55-56] nt |
| Conserved sequence flanking the nic site | CTGG|CTTA |
| Note | predicted by the oriTfinder |
Visualization of oriT structure Reference| ID | 485 |
| Name | Nuclease_pRK2 |
| GenBank accession number | WP_000635815 |
| Family | MOBP |
| Length | 517 aa |
| UniProt ID | _ |
| PDB ID | _ |
| Pfam | Relaxase [PF03432.13], Evalue: 4.90E-19, Aligned region: 49..246 |
| Note | putative relaxase |
| Protein sequence [Download] | MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF AEKELPPGGREKVMESFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRL QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLILPDNLPETKQALAESVTRGLEV LYHAGEIKGRQDVIQALTEVGLEVVRVTRSSISIADPNGGRNIRLKGAFYEQSFTDGRGV REKDERESRIYRENAERRIQQARRICQRGCDLKRDENQRRYSPVYGLDGGIAEKTPGRGE RGDDAAPEGHVKAGREFGHEVAGDSLSPVYREWRDALVSRREDTDEPGRNQGTGRDIAET EREDMGCGVCSGRGKKISGHSARDVSRGDALSGEGLDTTDGVIRNDRVGNTFAERLRAAA TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGTELERADRTLEPVIQREL EIREERLIQEREHALSLERERQPEIQERTLDGPSLGW |
Reference| ID | 875 |
| Plasmid name | pRK2 |
| GenBank accession number | NC_017661.1 |
| Genome size | 5360 bp |
| Coordinate of oriT [Strand] | 1742..1916 [-] |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Escherichia coli KO11FL [595495] |
Reference| [1] Turner PC et al (2012) Optical mapping and sequencing of the Escherichia coli KO11 genome reveal extensive chromosomal rearrangements, and multiple tandem copies of the Zymomonas mobilis pdc and adhB genes. J Ind Microbiol Biotechnol. 39(4):629-39. [PMID:22075923] |