oriTDB
The information of the oriT region
oriTDB accession number 100412
Name oriT_p12579_5  insolico
Sequence Completeness intact
oriT length 83 nt
oriT sequence GGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTA ACTATGTGGCATCAGTGCAGATT
IR (inverted repeat)[3-12] [16-25] nt  (GTGTCGGGGC..GCCATGACCC)
Location of nic site [56-57] nt
Conserved sequence flanking the
  nic site 		CTGG|CTTA
Note predicted by the oriTfinder
L:3-12;R:16-25;D:56-57
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 483
Name Nuclease_p12579_5 insolico
GenBank accession number WP_000635794
Family MOBP
Length 517 aa
UniProt ID _
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 7.80E-19, Aligned region: 49..246
Note putative relaxase
Protein sequence [Download] MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF
AEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQSGKRL
QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRILTLPDNLPETKQALAESVTRGIDA
LYHVGEIKGRQDVIQALTEAGLEVVRVTRSSISIADPNGGKNIRLKGAFYEQSFTDGRGV
REKAERESRIYRDNAERRVQQARKICKQGCDIKRDENQRRYSPVHSFDRGITEKTPGRGE
RGDDAAQEGRVKAGREYGHDVTGDGHFPVYREWRDALVSWRADTGEPGRNQDTGRNIAET
EREDMGRGVCAGREKEISGHPAGEIGNGDSLSGEGLGTTDGVTQSDGAGKTFAERLRATA
TGLYAAAERMGERLRGIAENVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREQ
EIRDERLIQEREHALSLERERQPEIQERTLDGPSLGW
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 873
Plasmid name p12579_5
GenBank accession number NC_017655.1
Incompatibility group ColRNAI
Genome size 5954 bp
Coordinate of oriT  [Strand] 3009..3091 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli O55:H7 str. RM12579 [1048689]
Reference
[1] Kyle JL et al (2012) Escherichia coli serotype O55:H7 diversity supports parallel acquisition of bacteriophage at Shiga toxin phage insertion sites during evolution of the O157:H7 lineage. J Bacteriol. 194(8):1885-96. [PMID:22328665]

Microbial Bioinformatics Group in MML, SJTU