oriTDB

The information of the oriT region

oriTDB accession number	100408
Name	oriT_pUMNK88_Ent
Sequence Completeness	intact
oriT length	464 nt
oriT sequence	AAGAAGAGCACCCCTAGCAGCGCCCCTAGGGTCATACTATAAAAAAACGCACGGCGCTGC TAGGGGCGGCCCTAATATATCCAAATGTTTTTCATAAAATTTGTCAGTACTGACCCTAAC AAGGGTCGCCATAGGGTCGCCATAGGGTCGTCAACGACCCTATTTAATAATGTAAAATAA ATTAAAATACATTATTTAAAACATAAGCTAATGATTCAAAAAGCAAATCAGCAAAAACTT GTTTTTTGCGTGGGGTGTGGTGCTTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTT TTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAAT GGATGTTAGCCATCTGCCTGATGTTTATAAATGAGATCTGCCAGGCCACTGATTGCTTTG ATCTTGCAGGCCGGGATTACAAAATAGATCCTGATTTACTGAGA
IR (inverted repeat)	[142-151] [154-163] nt (ATAGGGTCGT..ACGACCCTAT) [231-237] [243-249] nt (GCAAAAA..TTTTTGC)
Location of nic site	[258-259] nt
Conserved sequence flanking the nic site	GTGGGGTGT\|GG
Note	predicted by the oriTfinder
	L:142-151;R:154-163;L:231-237;R:243-249;D:258-259

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AAGAAGAGCACCCCTAGCAGCGCCCCTAGGGTCATACTATAAAAAAACGCACGGCGCTGC

61

TAGGGGCGGCCCTAATATATCCAAATGTTTTTCATAAAATTTGTCAGTACTGACCCTAAC

121

AAGGGTCGCCATAGGGTCGCCATAGGGTCGTCAACGACCCTATTTAATAATGTAAAATAA

181

ATTAAAATACATTATTTAAAACATAAGCTAATGATTCAAAAAGCAAATCAGCAAAAACTT

241

GTTTTTTGCGTGGGGTGTGGTGCTTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTT

301

TTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAAT

361

GGATGTTAGCCATCTGCCTGATGTTTATAAATGAGATCTGCCAGGCCACTGATTGCTTTG

421

ATCTTGCAGGCCGGGATTACAAAATAGATCCTGATTTACTGAGA

Reference
N/A

I. Information of Relaxase

ID	479
Name	TraI_pUMNK88_Ent
GenBank accession number	WP_000986925
Family	MOB_F
Length	1756 aa
UniProt ID	A0A0E0U825
PDB ID	_
Pfam	TrwC [PF08751.10], Evalue: 3.10E-74, Aligned region: 10..284 TraI [PF07057.10], Evalue: 6.80E-56, Aligned region: 1434..1556 AAA_30 [PF13604.5], Evalue: 8.40E-34, Aligned region: 968..1160 AAA_19 [PF13245.5], Evalue: 9.50E-09, Aligned region: 973..1108
Note	putative relaxase
Protein sequence [Download]	MMSIAQVRSAGSAGNYYSDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRL PDGADLSRMQDGRNRHRSGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVDFAARQVEAL ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKV GKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFSSRSQA IREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRDAADQRAE TRTQAPGPASPDGPDVQQAMTQAIAGLSERKVQFTYTDMLARTVGILPPENGVIERARAG IDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS DAVSVLAQERPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDE RLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQR TGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQ VSGVREQAILTQAIRRELKTQGVLGHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWN PETRSHDRYVIDRVTAQSNSLTLRDAQGETQVVRISSLDSSWSLFRQEKMPVADGERLRV TGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVGDSPFTALKLESGWVETPGH SVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI KARAGETLLETAISLQKAGLHTPAQQAIHLALPVVESKNLAFSHVDLLTEAKSFAAEGTS FADLGREINAQIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMER VPGELMEKLTSGQRAATRMLLETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRV VGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDM ARAYALIAAGAGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAV YSLINRDVERALSGLESVKPSQVPRQEGAWVPEHSVTEFSHSQEAKLAEAQQKAMLKGEA FPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM VPVLNTANIRDGELRRLSTWETHRDALVLVDNVYHRIAGISKDDGLITLEDAEGNTRLIS PREAVAEGVTLYTPDTIRVGTGDRMRFTMSDRERGYVANSVWTVTAVSGDSVTLSDGLQT RVIRPGQEQAEQHIDLAYAITAHGAQGASETFAIALEGTEGGRKQMAGFESAYVALSRMK QHVQVYTDERQGWTDAINNAVQKGTAHDVLEPKADREVMNAERLFSTARELRDVAAGRAV LRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGE GRVKGSWDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAIT GGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP DGKTEQAVREIAGQERDRAAITEREAALPESVLREPQREREAVREVARENLLQERLQQME RDMVRDLQKEKTLGGD

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	869
Plasmid name	pUMNK88_Ent
GenBank accession number	NC_017640.1
Incompatibility group	IncFIB(AP001918)
Genome size	81475 bp
Coordinate of oriT [Strand]	57013..57476 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli UMNK88 [696406]

Reference

[1] Shepard SM et al (2012) Genome sequences and phylogenetic analysis of K88- and F18-positive porcine enterotoxigenic Escherichia coli. J Bacteriol. 194(2):395-405. [PMID:22081385]