oriTDB
The information of the oriT region
oriTDB accession number 100408
Name oriT_pUMNK88_Ent  insolico
Sequence Completeness intact
oriT length 464 nt
oriT sequence AAGAAGAGCACCCCTAGCAGCGCCCCTAGGGTCATACTATAAAAAAACGCACGGCGCTGC TAGGGGCGGCCCTAATATATCCAAATGTTTTTCATAAAATTTGTCAGTACTGACCCTAAC AAGGGTCGCCATAGGGTCGCCATAGGGTCGTCAACGACCCTATTTAATAATGTAAAATAA ATTAAAATACATTATTTAAAACATAAGCTAATGATTCAAAAAGCAAATCAGCAAAAACTT GTTTTTTGCGTGGGGTGTGGTGCTTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTT TTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAAT GGATGTTAGCCATCTGCCTGATGTTTATAAATGAGATCTGCCAGGCCACTGATTGCTTTG ATCTTGCAGGCCGGGATTACAAAATAGATCCTGATTTACTGAGA
IR (inverted repeat)[142-151] [154-163] nt  (ATAGGGTCGT..ACGACCCTAT)
[231-237] [243-249] nt  (GCAAAAA..TTTTTGC)
Location of nic site [258-259] nt
Conserved sequence flanking the
  nic site 		GTGGGGTGT|GG
Note predicted by the oriTfinder
L:142-151;R:154-163;L:231-237;R:243-249;D:258-259
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 479
Name TraI_pUMNK88_Ent insolico
GenBank accession number WP_000986925
Family MOBF
Length 1756 aa
UniProt ID A0A0E0U825
PDB ID _
Pfam TrwC [PF08751.10], Evalue: 3.10E-74, Aligned region: 10..284
TraI [PF07057.10], Evalue: 6.80E-56, Aligned region: 1434..1556
AAA_30 [PF13604.5], Evalue: 8.40E-34, Aligned region: 968..1160
AAA_19 [PF13245.5], Evalue: 9.50E-09, Aligned region: 973..1108
Note putative relaxase
Protein sequence [Download] MMSIAQVRSAGSAGNYYSDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRL
PDGADLSRMQDGRNRHRSGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVDFAARQVEAL
ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKV
GKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFSSRSQA
IREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRDAADQRAE
TRTQAPGPASPDGPDVQQAMTQAIAGLSERKVQFTYTDMLARTVGILPPENGVIERARAG
IDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQERPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDE
RLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQR
TGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQ
VSGVREQAILTQAIRRELKTQGVLGHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWN
PETRSHDRYVIDRVTAQSNSLTLRDAQGETQVVRISSLDSSWSLFRQEKMPVADGERLRV
TGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVGDSPFTALKLESGWVETPGH
SVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKAGLHTPAQQAIHLALPVVESKNLAFSHVDLLTEAKSFAAEGTS
FADLGREINAQIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMER
VPGELMEKLTSGQRAATRMLLETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRV
VGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDM
ARAYALIAAGAGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAV
YSLINRDVERALSGLESVKPSQVPRQEGAWVPEHSVTEFSHSQEAKLAEAQQKAMLKGEA
FPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALVLVDNVYHRIAGISKDDGLITLEDAEGNTRLIS
PREAVAEGVTLYTPDTIRVGTGDRMRFTMSDRERGYVANSVWTVTAVSGDSVTLSDGLQT
RVIRPGQEQAEQHIDLAYAITAHGAQGASETFAIALEGTEGGRKQMAGFESAYVALSRMK
QHVQVYTDERQGWTDAINNAVQKGTAHDVLEPKADREVMNAERLFSTARELRDVAAGRAV
LRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGE
GRVKGSWDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAIT
GGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVREIAGQERDRAAITEREAALPESVLREPQREREAVREVARENLLQERLQQME
RDMVRDLQKEKTLGGD
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 869
Plasmid name pUMNK88_Ent
GenBank accession number NC_017640.1
Incompatibility group IncFIB(AP001918)
Genome size 81475 bp
Coordinate of oriT  [Strand] 57013..57476 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli UMNK88 [696406]
Reference
[1] Shepard SM et al (2012) Genome sequences and phylogenetic analysis of K88- and F18-positive porcine enterotoxigenic Escherichia coli. J Bacteriol. 194(2):395-405. [PMID:22081385]

Microbial Bioinformatics Group in MML, SJTU