oriTDB
The information of the oriT region
oriTDB accession number 100406
Name oriT_pRK2  insolico
Sequence Completeness intact
oriT length 175 nt
oriT sequence GGTTTCGGGGCGAAGCCCTGAACCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAC TATGTTGGCATGGATGCGGAAATCAGTGAAGTGCTTCATGTGACAGGAGAAAAAAGGCGG CACAGGCGCGTCAGCAGAATATGCGATACAGGATATATTCCGCTTCCTCGCTCAC
IR (inverted repeat)[2-11] [15-24] nt  (GTTTCGGGGC..GCCCTGAACC)
Location of nic site [55-56] nt
Conserved sequence flanking the
  nic site 		CTGG|CTTA
Note predicted by the oriTfinder
L:2-11;R:15-24;D:55-56
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 477
Name Nuclease_pRK2 insolico
GenBank accession number WP_000635814
Family MOBP
Length 517 aa
UniProt ID _
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 4.80E-19, Aligned region: 49..246
Note putative relaxase
Protein sequence [Download] MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF
AEKELPPGGREKVMESFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRL
QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLILPDNLPETKQALAESVTRGLEV
LYHAGEIKGRQDVIQALTEVGLEVVRVTRSSISIADPNGGRNIRLKGAFYEQSFTDGRGV
REKAERESRIYRENAERRIQQARRICQRGCDLKRDENQRRYSPVYGLDGGIAEKTPGRGE
RGDDAAPEGHVKAGREFGHEVAGDSLSPVYREWRDALVSRREDTDEPGRNQGTGRDIAET
EREDMGCGVCSGRGKKISGHSARDVSRGDALSGEGLDTTDGVIRNDRVGNTFAERLRAAA
TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGTELERADRTLEPVIQREL
EIREERLIQEREHALSLERERQPEIQERTLDGPSLGW
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 867
Plasmid name pRK2
GenBank accession number NC_017636.1
Genome size 5360 bp
Coordinate of oriT  [Strand] 1742..1916 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli W [566546]
Reference
[1] Archer CT et al (2011) The genome sequence of E coli W (ATCC 9637): comparative genome analysis and an improved genome-scale reconstruction of E coli. BMC Genomics. 12:09. [PMID:21208457]

Microbial Bioinformatics Group in MML, SJTU