oriTDB
The information of the oriT region
oriTDB accession number 100403
Name oriT_pCV56B  insolico
Sequence Completeness intact
oriT length 463 nt
oriT sequence TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGGA AAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTCTTTTTGTATGACA AAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAAA GCAAATCGACACACTCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGC CACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTGCAGTATTTAT GGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAAGGCGCAATGCTTATTA CAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGAG GCTATTTATGGATCAGAAAAATGTATCACAAAATCAAACAAAG
IR (inverted repeat)IR1: [138-147] [152-161] nt  (CCCATTTTAT..ATAAAATGGG)
IR2: [207-219] [223-235] nt  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
IR3: [242-255] [268-281] nt  (ACATTGTAATACAA..TTGTATTACAATGT)
Location of nic site [290-291] nt
Conserved sequence flanking the
  nic site 		CTTG|CA
Note predicted by the oriTfinder
L:138-147;R:152-161;L:207-219;R:223-235;L:242-255;R:268-281;D: 290-291
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 474
Name Relaxase_pCV56B insolico
GenBank accession number WP_014570908
Family MOBP
Length 410 aa
UniProt ID _
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 4.00E-75, Aligned region: 9..280
Note putative relaxase
Protein sequence [Download] MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGI
DPFIAKEQFDVVRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLY
PNHQSAVYTHLDGKNHVLHNHIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWH
ILEPPKERQTETEKELIAKNEYSYMDDLRERINKSLQDVSVSSYETFKERLSDNGVILSE
RGQTFSYAFLDANNKQRRARETRLGSDFGKETILHELENRARQNEFSAVEQREPAITPLE
RDTQQRESEIVSLEQAIEPRKSEARKRESKINRFIDTIKQFAGRVPELTQRVTRKLKQTK
EKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSL
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 864
Plasmid name pCV56B
GenBank accession number NC_017487.1
Genome size 35934 bp
Coordinate of oriT  [Strand] 32692..33154 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Lactococcus lactis subsp. lactis CV56 [929102]
Reference
[1] Gao Y et al (2011) Complete genome sequence of Lactococcus lactis subsp lactis CV56, a probiotic strain isolated from the vaginas of healthy women. J Bacteriol. 193(11):2886-7. [PMID:21460077]

Microbial Bioinformatics Group in MML, SJTU