oriTDB

The information of the oriT region

oriTDB accession number	100402
Name	oriT_pCV56C
Sequence Completeness	intact
oriT length	463 nt
oriT sequence	TTAGTGGATGAACAAAAAAATTCGAAAGATTTTTTGTTCTTTCATCCATGATTTTAGAAA AAAGAGGGACGATTTCAGAAGACGAAATTCGTCTCTTTTTTTCTTCTTTTTGTATGACAA AAAGAAATATCTTTTGCCCATTTTATTTTTATCAAATGGGCAGCTGGCGTTTGCGTAAAG CAAATCAACACAATCCAAAGGGGATAAAAGTGGAAAGTGAAACTTCCCCCTTTTCAAGCC ACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTAATAGCTTACCAGTATTTAT GGTTTTATATTTTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATTA CAAAATCAATGACAAAGGGCGATTGACTAATGAGCGCTGAGGCATTTTATCTTTGAGGAA GTTCTTGATGGATCAGAAAAATGTATCACAAATTTAAACAAAG
IR (inverted repeat)	IR1: [137-146] [151-160] nt (CCCATTTTAT..ATCAAATGGG) IR2: [206-218] [222-234] nt (AAAAGTGGAAAGT..ACTTCCCCCTTTT) IR3: [241-254] [267-280] nt (ACATTGTAATACAA..TTGTATTACAATGT)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	predicted by the oriTfinder
	L:137-146;R:151-160;L:206-218;R:222-234;L:241-254;R:267-280

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TTAGTGGATGAACAAAAAAATTCGAAAGATTTTTTGTTCTTTCATCCATGATTTTAGAAA

61

AAAGAGGGACGATTTCAGAAGACGAAATTCGTCTCTTTTTTTCTTCTTTTTGTATGACAA

121

AAAGAAATATCTTTTGCCCATTTTATTTTTATCAAATGGGCAGCTGGCGTTTGCGTAAAG

181

CAAATCAACACAATCCAAAGGGGATAAAAGTGGAAAGTGAAACTTCCCCCTTTTCAAGCC

241

ACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTAATAGCTTACCAGTATTTAT

301

GGTTTTATATTTTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATTA

361

CAAAATCAATGACAAAGGGCGATTGACTAATGAGCGCTGAGGCATTTTATCTTTGAGGAA

421

GTTCTTGATGGATCAGAAAAATGTATCACAAATTTAAACAAAG

Reference
N/A

I. Information of Relaxase

ID	473
Name	PriA_pCV56C
GenBank accession number	WP_014570287
Family	MOB_P
Length	505 aa
UniProt ID	_
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.90E-50, Aligned region: 16..262
Note	putative relaxase
Protein sequence [Download]	MTVIYMPKQSNGTVHSSKDLNQLIDYVMNPEKTNDFEYVSGQNILDIHSTCDEMLATRTM ANALKNKPQKNEIFGYHFVQSFSPDDHLTPEQVHEIGLKTMKEYLGSSAEFIIATHTDKP HLHNHIVLNATDPLTLNKFQQNKNDLERLKEISDKISKEYGCKIIVRPEQKLGNSHKNYL VYLAKNSYRKEIKNKLEFLMNHSHTWEDFKTKARALNLKVDDTKKYTTYLLEGSEQTKKI RDRSLKNDKFLKENLKERIEKNTIGYSVDEVVKLWKDKESIQEKNQEKEISILVEDWQIE KETENFLYVTIDTALDKEATIKIPARCVDKLENGDYQVFIKNKDRFSYLDKKSPANNKIM YGTTVAKNLQRQSGNVPLYSDNANIKLKQVFHEFDFLISQGLGFDRSFETIGKDLKATYQ ETEKQLDKLDTKIIEYVEQNKSLDYVDTTIRDTIKNLKLERDDLRDTLYKVDKNIQYYQK SEQRLEAYQKNQSPKNKARDDDFEI

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	863
Plasmid name	pCV56C
GenBank accession number	NC_017484.1
Genome size	31442 bp
Coordinate of oriT [Strand]	23520..23982 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Lactococcus lactis subsp. lactis CV56 [929102]

Reference

[1] Gao Y et al (2011) Complete genome sequence of Lactococcus lactis subsp lactis CV56, a probiotic strain isolated from the vaginas of healthy women. J Bacteriol. 193(11):2886-7. [PMID:21460077]