oriTDB

The information of the oriT region

oriTDB accession number	100401
Name	oriT_pEp5
Sequence Completeness	intact
oriT length	182 nt
oriT sequence	GTTTTAGGGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATA CTGGCTTAGTATGTTGCCACGGCGTTCTGTTTCATGGAAGTGCTTCATGTGGTGGTGAAA AAGGCGGATTCGGAGTGACAGGGGAATATGCAGGCTCAGGTGATATGACGCCTCCTCGTT CA
IR (inverted repeat)	[13-20] [24-31] nt (TTCGGGGC..GCCCTGAA)
Location of nic site	[64-65] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:13-20;R:24-31;D:64-65

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GTTTTAGGGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATA

61

CTGGCTTAGTATGTTGCCACGGCGTTCTGTTTCATGGAAGTGCTTCATGTGGTGGTGAAA

121

AAGGCGGATTCGGAGTGACAGGGGAATATGCAGGCTCAGGTGATATGACGCCTCCTCGTT

181

CA

Reference
N/A

I. Information of Relaxase

ID	472
Name	Nuclease_pEp5
GenBank accession number	WP_012814659
Family	MOB_P
Length	504 aa
UniProt ID	D2TE66
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 2.40E-15, Aligned region: 47..247
Note	putative relaxase
Protein sequence [Download]	MIVKFHPRGRGGGSGPVDYLLGKDRQRDGASVLQGKPEEVLELIDASAYAKKYTSGVLSF AEKDLAPGQREKLMASFERVLMPGLDKDQYSVLWVEHQDKGRLELNFLIPNTELLTGRRL QPYYDRADRPRIDAWQTVVNGRLGLHDPNAPENRRALVTPSALPKAKLEAAETITRGLLA IASSGELKTRQDVTEALESAGFDVVRTTKSSISIADPDGGRNIRLKGAIYEQSFNAGEGL RAEIESAAAEYRRDAESRIQRAREVCKSGTERKREENQRRHSRPRPAAVLSHEPAHERHG THGQPDVADHRPGFRPVDRLERGHSVVAGAADTRQLHEHPGTEGRAGEAERADMGREISG RQPGAFSGAAGRRESGHELDVRQRQEERHQTGTGVEQHDGAGKTVAERIRAATAGLLEKA GRVGERLRGMADDVWSYATGERGAERARHGFEQAGAEFERAAAPVVVKLNGIEAHRQQER AIQQQKELELERSQRQRTYDGPSL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	862
Plasmid name	pEp5
GenBank accession number	NC_017389.1
Genome size	4960 bp
Coordinate of oriT [Strand]	4305..4486 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Erwinia pyrifoliae DSM 12163 [644651]

Reference

[1] Smits TH et al (2010) Complete genome sequence of the fire blight pathogen Erwinia pyrifoliae DSM 12163T and comparative genomic insights into plant pathogenicity. BMC Genomics. 0.459722222. [PMID:20047678]