oriTDB
The information of the oriT region
oriTDB accession number 100401
Name oriT_pEp5  insolico
Sequence Completeness intact
oriT length 182 nt
oriT sequence GTTTTAGGGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATA CTGGCTTAGTATGTTGCCACGGCGTTCTGTTTCATGGAAGTGCTTCATGTGGTGGTGAAA AAGGCGGATTCGGAGTGACAGGGGAATATGCAGGCTCAGGTGATATGACGCCTCCTCGTT CA
IR (inverted repeat)[13-20] [24-31] nt  (TTCGGGGC..GCCCTGAA)
Location of nic site [64-65] nt
Conserved sequence flanking the
  nic site 		CTGG|CTTA
Note predicted by the oriTfinder
L:13-20;R:24-31;D:64-65
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 472
Name Nuclease_pEp5 insolico
GenBank accession number WP_012814659
Family MOBP
Length 504 aa
UniProt ID D2TE66
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 2.40E-15, Aligned region: 47..247
Note putative relaxase
Protein sequence [Download] MIVKFHPRGRGGGSGPVDYLLGKDRQRDGASVLQGKPEEVLELIDASAYAKKYTSGVLSF
AEKDLAPGQREKLMASFERVLMPGLDKDQYSVLWVEHQDKGRLELNFLIPNTELLTGRRL
QPYYDRADRPRIDAWQTVVNGRLGLHDPNAPENRRALVTPSALPKAKLEAAETITRGLLA
IASSGELKTRQDVTEALESAGFDVVRTTKSSISIADPDGGRNIRLKGAIYEQSFNAGEGL
RAEIESAAAEYRRDAESRIQRAREVCKSGTERKREENQRRHSRPRPAAVLSHEPAHERHG
THGQPDVADHRPGFRPVDRLERGHSVVAGAADTRQLHEHPGTEGRAGEAERADMGREISG
RQPGAFSGAAGRRESGHELDVRQRQEERHQTGTGVEQHDGAGKTVAERIRAATAGLLEKA
GRVGERLRGMADDVWSYATGERGAERARHGFEQAGAEFERAAAPVVVKLNGIEAHRQQER
AIQQQKELELERSQRQRTYDGPSL
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 862
Plasmid name pEp5
GenBank accession number NC_017389.1
Genome size 4960 bp
Coordinate of oriT  [Strand] 4305..4486 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Erwinia pyrifoliae DSM 12163 [644651]
Reference
[1] Smits TH et al (2010) Complete genome sequence of the fire blight pathogen Erwinia pyrifoliae DSM 12163T and comparative genomic insights into plant pathogenicity. BMC Genomics. 0.459722222. [PMID:20047678]

Microbial Bioinformatics Group in MML, SJTU