oriTDB

The information of the oriT region

oriTDB accession number	100397
Name	oriT_pUUH239.1
Sequence Completeness	intact
oriT length	180 nt
oriT sequence	TTAGCGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTG GCTTATTATGTTGGCATGAGTGCGGTAATCAGGAAAGTGCTTCATGTGACAGAGGAAAAT GCAGCTGCGGGCGTAAGGCAGAAAATGGAAGTTCAGAGGATATGACGCCTCCTCGCTCAC
IR (inverted repeat)	[10-17] [21-28] nt (TTCGGGGC..GCCCTGAA)
Location of nic site	[61-62] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:10-17;R:21-28;D:61-62

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TTAGCGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTG

61

GCTTATTATGTTGGCATGAGTGCGGTAATCAGGAAAGTGCTTCATGTGACAGAGGAAAAT

121

GCAGCTGCGGGCGTAAGGCAGAAAATGGAAGTTCAGAGGATATGACGCCTCCTCGCTCAC

Reference
N/A

I. Information of Relaxase

ID	468
Name	Nuclease_pUUH239.1
GenBank accession number	YP_005352162
Family	MOB_P
Length	501 aa
UniProt ID	G7RUW6
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 4.90E-16, Aligned region: 48..246
Note	putative relaxase
Protein sequence [Download]	MIVKFHPRGRGGGAGPVDYCWEKTAARGGDRAAGKTGGSPGAIDASPYAKKYTSGVLSFA EAELPPGQREQIMASFERVLMPGLDKDQYSILWVEHTDKGRLELNFLIPNTELLTGKRLQ PYYDRADRPRIDAWQTVVNGRLGLHDPNAPENRRLLVTPSALPETKLEAAQAITRGLLAL ASSGELKTREDVTGALTAAGFEVVRTTKNSISIADPDGGRNIRLKGAIYEQSFSAGEGLG AAIESAAAEYRRDAESRIQRARAVCKSGTERKREENQRRHQRPRAGYDRGDAVEPAERAA YGRPDVADHHPGLGTADRHEREHSVVAGAADHRELPDYPGAGADAGHAVGEEPRRAAVAL RGEETVLRQSEPESGSLRGGRELDGTGEEIAHDGTGKTVADRIRAATSGLLAKAGRVGER LRGMAEDVWSYATGERGAERARHALESAGGELEQAVTAFEPVVQRHEMAVAAERAYEQKQ HEKELAETRSRQRSYDGPTLG

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	858
Plasmid name	pUUH239.1
GenBank accession number	NC_016979.1
Genome size	5247 bp
Coordinate of oriT [Strand]	4957..5136 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Klebsiella pneumoniae U-0608239 [573]

Reference

[1] Sandegren L et al (2012) Transfer of an Escherichia coli ST131 multiresistance cassette has created a Klebsiella pneumoniae-specific plasmid associated with a major nosocomial outbreak. J Antimicrob Chemother. 67(1):74-83. [PMID:21990049]