oriTDB
The information of the oriT region
oriTDB accession number 100397
Name oriT_pUUH239.1  insolico
Sequence Completeness intact
oriT length 180 nt
oriT sequence TTAGCGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTG GCTTATTATGTTGGCATGAGTGCGGTAATCAGGAAAGTGCTTCATGTGACAGAGGAAAAT GCAGCTGCGGGCGTAAGGCAGAAAATGGAAGTTCAGAGGATATGACGCCTCCTCGCTCAC
IR (inverted repeat)[10-17] [21-28] nt  (TTCGGGGC..GCCCTGAA)
Location of nic site [61-62] nt
Conserved sequence flanking the
  nic site
CTGG|CTTA
Note predicted by the oriTfinder
L:10-17;R:21-28;D:61-62
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 468
Name Nuclease_pUUH239.1 insolico
GenBank accession number YP_005352162
Family MOBP
Length 501 aa
UniProt ID G7RUW6
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 4.90E-16, Aligned region: 48..246
Note putative relaxase
Protein sequence [Download] MIVKFHPRGRGGGAGPVDYCWEKTAARGGDRAAGKTGGSPGAIDASPYAKKYTSGVLSFA
EAELPPGQREQIMASFERVLMPGLDKDQYSILWVEHTDKGRLELNFLIPNTELLTGKRLQ
PYYDRADRPRIDAWQTVVNGRLGLHDPNAPENRRLLVTPSALPETKLEAAQAITRGLLAL
ASSGELKTREDVTGALTAAGFEVVRTTKNSISIADPDGGRNIRLKGAIYEQSFSAGEGLG
AAIESAAAEYRRDAESRIQRARAVCKSGTERKREENQRRHQRPRAGYDRGDAVEPAERAA
YGRPDVADHHPGLGTADRHEREHSVVAGAADHRELPDYPGAGADAGHAVGEEPRRAAVAL
RGEETVLRQSEPESGSLRGGRELDGTGEEIAHDGTGKTVADRIRAATSGLLAKAGRVGER
LRGMAEDVWSYATGERGAERARHALESAGGELEQAVTAFEPVVQRHEMAVAAERAYEQKQ
HEKELAETRSRQRSYDGPTLG
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 858
Plasmid name pUUH239.1
GenBank accession number NC_016979.1
Genome size 5247 bp
Coordinate of oriT  [Strand] 4957..5136 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Klebsiella pneumoniae U-0608239 [573]
Reference
[1] Sandegren L et al (2012) Transfer of an Escherichia coli ST131 multiresistance cassette has created a Klebsiella pneumoniae-specific plasmid associated with a major nosocomial outbreak. J Antimicrob Chemother. 67(1):74-83. [PMID:21990049]